Quan Mengqiu, Li Jingrui, Cui Minghui, Sha Genzheng, Wang Yuqing, Wu Bozhen, Zhu Jin, Chen Jing
College of Materials Science and Engineering, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR China; Key Laboratory of Bio-based Polymeric Materials Technology and Application of Zhejiang Province, Laboratory of Polymers and Composites, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Zhejiang, Ningbo 315201, PR China.
Key Laboratory of Bio-based Polymeric Materials Technology and Application of Zhejiang Province, Laboratory of Polymers and Composites, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Zhejiang, Ningbo 315201, PR China.
Int J Biol Macromol. 2025 Feb;288:138733. doi: 10.1016/j.ijbiomac.2024.138733. Epub 2024 Dec 16.
Wound healing is a complex process and the mechanism of the tissue repair process involves many complex steps: inflammation, proliferation, and maturation. Wounds can be divided into two main categories: acute and chronic wounds. Non-healing wounds usually follow a bacterial infection. Many types of materials on the market have been developed for use as wound dressings. Polyurethane foam for wound dressings has many advantages over other materials, especially for moderate wounds and drainage. In this study, lignin-based non-isocyanate polyurethane foams were prepared using a green route by oxygen alkylation modification of enzymatically dissolved lignin, cyclic carbonation and polymerization with diamines to add a blowing agent. By loading CuO on the surface of the prepared lignin-based non-isocyanate polyurethane foam, a pH-responsive wound dressing, named lignin-based non-isocyanate polyurethane/copper peroxide composite foam (named NIPU foam-CuO), was prepared, which can specifically release the strong oxidizing OH under acidic conditions. The composite foam can effectively kill the bacteria in the wound. The test results proved that the composite foam has excellent mechanical properties, thermal stability, and biocompatibility. NIPU-foam-CuO 100 mM inhibited two types of bacteria, Escherichia coli, and Staphylococcus aureus, by up to 98 % and 95 % within 8 h, respectively. It also shows excellent performance in promoting wound healing in organism experiments as well as in the subsequent histological staining. The lignin-based NIPU foams of this work exhibit remarkable innovation and unique properties in terms of environmental friendliness, performance and antimicrobial resistance. At the same time, we also mention potential problems such as drug resistance in the long-term use of NIPU-CuO foams.
伤口愈合是一个复杂的过程，组织修复过程的机制涉及许多复杂步骤：炎症、增殖和成熟。伤口可分为两大类：急性伤口和慢性伤口。不愈合伤口通常继发细菌感染。市场上已开发出多种材料用作伤口敷料。用于伤口敷料的聚氨酯泡沫比其他材料具有许多优势，特别是对于中度伤口和引流。在本研究中，通过对酶解木质素进行氧烷基化改性、环碳酸化以及与二胺聚合以添加发泡剂，采用绿色路线制备了木质素基非异氰酸酯聚氨酯泡沫。通过在制备的木质素基非异氰酸酯聚氨酯泡沫表面负载氧化铜，制备了一种pH响应性伤口敷料，命名为木质素基非异氰酸酯聚氨酯/过氧化铜复合泡沫（简称NIPU泡沫 - CuO），其在酸性条件下能特异性释放强氧化性的羟基自由基。该复合泡沫能有效杀灭伤口中的细菌。测试结果证明该复合泡沫具有优异的力学性能、热稳定性和生物相容性。100 mM的NIPU - 泡沫 - CuO在8小时内分别对大肠杆菌和金黄色葡萄球菌两种细菌的抑制率高达98%和95%。在生物体实验以及后续组织学染色中，它在促进伤口愈合方面也表现出优异性能。本工作中的木质素基NIPU泡沫在环境友好性、性能和抗菌性方面展现出显著的创新性和独特性能。同时，我们也提到了NIPU - CuO泡沫长期使用中潜在的耐药性等问题。
