Vieira Raquel S F, Venâncio Carlos A S, Félix Luís M
Centre for the Research and Technology of Agro-Environment and Biological Sciences (CITAB), University of Trás-Os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
Inov4Agro, Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, University of Trás-Os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
Fish Physiol Biochem. 2025 Feb;51(1):25. doi: 10.1007/s10695-024-01421-7. Epub 2024 Dec 14.
Animal welfare is a growing concern in aquaculture practices. Stress induced by handling or transportation can lead to negative impacts on the sector. Zebrafish has raised as an important aquaculture model, but still with few focus on its stress response in early life stages. Therefore, the objective of this study was to improve the evaluation of different markers of the stress response after a stress event in a zebrafish larvae model. Zebrafish larvae (96 hpf) were vortex-stimulated for 1 min at 200 rpm for acute stress induction. After 10 min, 1- and 4-h behavioral larvae outcomes and larvae were sampled to the following quantification: levels of cortisol, lactate, glucose and biochemical biomarkers (reactive oxygen species, superoxide dismutase, catalase, glutathione peroxidase, lipidic oxidation level and protein carbonylation, glutathione s-transferase, acetylcholinesterase, lactate dehydrogenase and ATPase), and the metabolic rate. The cortisol, glucose, and lactate levels had no alterations. At the behavioral level, an increase in the distance swam and in the speed was observed and the metabolic rate also increased according to the behavioral outcomes. The ATPase and GST activity showed a decrease in their activity, probably through osmoregulation changes related to the hypothetic adrenocorticotropic hormone downregulation. Overall, the acute vortex stimulation at low speed induced an early stress response independent of the HPI-cortisol pathway. In addition, this study shows zebrafish early life stages as a sensitive model to acute vortex stimulation, identifying altered parameters which can be used in future work to assess the effect on animal welfare in similar acute situations.
动物福利在水产养殖实践中日益受到关注。处理或运输引起的应激会对该行业产生负面影响。斑马鱼已成为一种重要的水产养殖模型，但在其早期生命阶段的应激反应方面仍很少受到关注。因此，本研究的目的是改进对斑马鱼幼体模型应激事件后应激反应不同标志物的评估。对斑马鱼幼体（96小时胚胎期）以200转/分钟进行1分钟的涡旋刺激以诱导急性应激。10分钟后，对幼体1小时和4小时的行为结果进行观察，并采集幼体进行以下量化分析：皮质醇、乳酸、葡萄糖水平以及生化生物标志物（活性氧、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、脂质氧化水平和蛋白质羰基化、谷胱甘肽S-转移酶、乙酰胆碱酯酶、乳酸脱氢酶和ATP酶），以及代谢率。皮质醇、葡萄糖和乳酸水平没有变化。在行为层面，观察到游动距离和速度增加，并且代谢率也根据行为结果而增加。ATP酶和GST活性显示其活性降低，可能是由于与假定的促肾上腺皮质激素下调相关的渗透调节变化。总体而言，低速急性涡旋刺激诱导了独立于下丘脑-垂体-肾上腺皮质醇途径的早期应激反应。此外，本研究表明斑马鱼早期生命阶段是对急性涡旋刺激敏感的模型，确定了可用于未来工作中评估类似急性情况下对动物福利影响的参数变化。
