Firouzabadi Amir Masoud, Imani Maryam, Tofighi Niaki Maryam, Fesahat Farzaneh
Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Health Reproductive Research Center, Sari Branch, Islamic Azad University, Sari, Iran.
Am J Mens Health. 2024 Nov-Dec;18(6):15579883241306893. doi: 10.1177/15579883241306893.
Antioxidants act by preventing excessive oxidative stress within the developing sperm cells. Hookah smoking has been implicated in inducing oxidative stress which may be a risk factor for male infertility. This study aims to bridge this knowledge gap by evaluating the effect of hookah smoking on sperm quality and the expression of () and 1 () genes which are involved in antioxidant response. Young fertile men in reproductive age were categorized into hookah smokers ( = 32) and non-smokers (n=52). Sperm parameters (concentration, volume, motility, morphology) as well as gene expression of and were evaluated in each sperm sample. There was no meaningful change in sperm parameters in the case group compared with the controls. The mRNA levels of was significantly higher in the case group compared with the controls ( = 0.01), while expression did not significantly change between the study groups. A remarkable increase in expression in hookah smokers might be a cellular defense mechanism, indicating activation of antioxidant response in sperm to counteract potential oxidative stress caused by hookah use. However, further research is needed to understand the functional consequences of this upregulation.
抗氧化剂通过防止发育中的精子细胞内出现过度氧化应激来发挥作用。水烟吸食与诱导氧化应激有关，而氧化应激可能是男性不育的一个风险因素。本研究旨在通过评估水烟吸食对精子质量以及参与抗氧化反应的（）和1（）基因表达的影响，来填补这一知识空白。将处于生育年龄的年轻有生育能力的男性分为水烟吸食者（=32）和非吸烟者（n = 52）。对每个精子样本评估精子参数（浓度、体积、活力、形态）以及和的基因表达。与对照组相比，病例组的精子参数没有显著变化。与对照组相比，病例组中的mRNA水平显著更高（=0.01），而研究组之间的表达没有显著变化。水烟吸食者中表达的显著增加可能是一种细胞防御机制，表明精子中的抗氧化反应被激活，以抵消水烟使用引起的潜在氧化应激。然而，需要进一步研究来了解这种上调的功能后果。
