Bhandari S, Kuehne S, Camilleri J
Dentistry, School of Health Sciences, College of Medicine and Health, University of Birmingham, Birmingham, UK.
School of Science & Technology, Nottingham Trent University, Nottingham, UK.
J Dent Res. 2025 Mar;104(3):260-269. doi: 10.1177/00220345241287504. Epub 2024 Dec 14.
Root canal obturation involves filling of the chemomechanically prepared root canal space. Despite reduced microbial load, residual bacteria can still lead to reinfection and treatment failure. Currently, obturation techniques use a combination of gutta-percha and sealer, which requires the preparation of the root canal to specific sizes and tapers to enable the fitting of customized cones. This study aims to characterize the physical, chemical, and antimicrobial properties of a new light-curable injectable material (OdneFill, Switzerland) used to obturate the root canal. Odnefill and 2 root canal sealers (AH Plus and BioRoot RCS) were characterized by scanning electron microscopy (SEM) and energy-dispersive spectroscopy following exposure to chlorhexidine, sodium hypochlorite, and water. The flow, film thickness, radiopacity, solubility, and contact angle were evaluated. The susceptibility to microbial degradation was assessed by weight changes after contact with bacterial enzymes (lipase and cholesterol esterase). A multispecies biofilm composed of , , , and was used to assess changes to the material microstructure (SEM). Further, bacterial viability in contact with the materials was evaluated using live/dead staining and confocal microscopy. A direct contact assay was carried out, and the utilization of the materials as a carbon source for the bacterial biofilm was also assessed. Statistical analysis was performed using 1-way analysis of variance and Tukey post hoc tests ( = 0.05). OdneFill was composed of an organic matrix with zirconium oxide filler. It exhibited comparable physical properties to AH Plus and BioRoot RCS and was stable in contact with irrigating solutions and with the bacterial enzymes (cholesterol esterase and lipase). Its antimicrobial characteristics were better than those of AH Plus when placed in contact with a multispecies biofilm. Based on the findings, OdneFill presents itself as suitable root canal-filling material and warrants further clinical investigation.
根管充填包括对经化学机械预备的根管空间进行充填。尽管微生物负荷降低，但残留细菌仍可导致再感染和治疗失败。目前，充填技术使用牙胶和封闭剂的组合，这需要将根管预备成特定的尺寸和锥度，以便能适配定制的牙胶尖。本研究旨在表征一种用于根管充填的新型光固化可注射材料（瑞士的OdneFill）的物理、化学和抗菌性能。通过扫描电子显微镜（SEM）和能谱分析对OdneFill以及两种根管封闭剂（AH Plus和BioRoot RCS）在接触氯己定、次氯酸钠和水后的情况进行表征。评估了其流动性、膜厚度、射线不透性、溶解性和接触角。通过与细菌酶（脂肪酶和胆固醇酯酶）接触后的重量变化评估其对微生物降解的敏感性。使用由[具体细菌种类]组成的多菌种生物膜来评估材料微观结构的变化（SEM）。此外，使用活/死染色和共聚焦显微镜评估与材料接触时细菌的活力。进行了直接接触试验，并评估了材料作为细菌生物膜碳源的利用情况。使用单因素方差分析和Tukey事后检验进行统计分析（α = 0.05）。OdneFill由含有氧化锆填料的有机基质组成。它表现出与AH Plus和BioRoot RCS相当的物理性能，并且在与冲洗液和细菌酶（胆固醇酯酶和脂肪酶）接触时稳定。当与多菌种生物膜接触时，其抗菌特性优于AH Plus。基于这些发现，OdneFill表现为一种合适的根管充填材料，值得进一步的临床研究。
