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大鼠胰岛肾被膜下隔离与移植治疗糖尿病的方案

Protocol for isolation and transplantation of rat pancreatic islets in the kidney capsule to treat diabetes.

作者信息

Li Dayan, Kong Weijing, Hong Yi, Zhang Wei, Sun Bingbing, Wang Xi, Wang Kai

机构信息

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Clinical Stem Cell Research Center, Peking University Third Hospital, Peking University, Beijing 100191, China.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Clinical Stem Cell Research Center, Peking University Third Hospital, Peking University, Beijing 100191, China.

出版信息

STAR Protoc. 2025 Mar 21;6(1):103486. doi: 10.1016/j.xpro.2024.103486. Epub 2024 Dec 13.

DOI:10.1016/j.xpro.2024.103486
PMID:39673701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11699416/
Abstract

Islet transplantation offers promise for those with type 1 and late-stage type 2 diabetes; however, islet isolation from the pancreas is challenging due to the interference of exocrine tissue. We describe steps for constructing a diabetes model and precise islet isolation by a perfusion-based technique, followed by gradient centrifugation and cultivation in RPMI 1640 for recovery. Transplanted rat islets in diabetic nude mice demonstrated functional recovery, confirming the effectiveness of this approach.

摘要

胰岛移植为1型和晚期2型糖尿病患者带来了希望；然而，由于外分泌组织的干扰，从胰腺中分离胰岛具有挑战性。我们描述了构建糖尿病模型和通过基于灌注的技术精确分离胰岛的步骤，随后进行梯度离心，并在RPMI 1640中培养以恢复胰岛功能。将大鼠胰岛移植到糖尿病裸鼠体内显示出功能恢复，证实了该方法的有效性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/107d090eb2db/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/026400b0f172/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/4e8ec8d14a5c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/db797f30c4f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/b80c60f9dca1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/518535430800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/107d090eb2db/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/026400b0f172/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/4e8ec8d14a5c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/db797f30c4f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/b80c60f9dca1/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/518535430800/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/85bc/11699416/107d090eb2db/gr5.jpg

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1
Protocol for isolation and transplantation of rat pancreatic islets in the kidney capsule to treat diabetes.大鼠胰岛肾被膜下隔离与移植治疗糖尿病的方案
STAR Protoc. 2025 Mar 21;6(1):103486. doi: 10.1016/j.xpro.2024.103486. Epub 2024 Dec 13.
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[Isogenic islet transplantation on the rat liver (method for isolation and purification of the Langerhans islets)].大鼠肝脏同种胰岛移植（胰岛分离与纯化方法）
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[Isolation, purification, and functional evaluation of human pancreatic islet].人胰岛的分离、纯化及功能评估
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Rescue purification maximizes the use of human islet preparations for transplantation.挽救性纯化可最大限度地利用人类胰岛制剂进行移植。
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Islet cell transplantation: in vivo and in vitro functional assessment of nonhuman primate pancreatic islets.胰岛移植：非人灵长类动物胰岛的体内和体外功能评估
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A prospective comparison of discontinuous EuroFicoll and EuroDextran gradients for islet purification.用于胰岛纯化的间断式欧洲菲可（EuroFicoll）和欧洲葡聚糖（EuroDextran）梯度的前瞻性比较。
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Xenotransplantation of layer-by-layer encapsulated non-human primate islets with a specified immunosuppressive drug protocol.层状包裹非人灵长类胰岛细胞的异种移植与特定免疫抑制药物方案。
J Control Release. 2017 Jul 28;258:10-21. doi: 10.1016/j.jconrel.2017.04.021. Epub 2017 Apr 19.

本文引用的文献

1
Protocol for transplanting pancreatic islets into the parametrial fat pad of female mice.将胰腺胰岛移植到雌性小鼠的宫旁脂肪垫中的方案。
STAR Protoc. 2024 Mar 15;5(1):102816. doi: 10.1016/j.xpro.2023.102816. Epub 2024 Jan 4.
2
A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model.一种改良的手术方法，使用微创回结肠静脉灌注在小鼠肝内胰岛移植模型中。
STAR Protoc. 2022 May 19;3(2):101416. doi: 10.1016/j.xpro.2022.101416. eCollection 2022 Jun 17.
3
Protocol for in vivo and ex vivo assessments of glucose-stimulated insulin secretion in mouse islet β cells.
用于在体和离体评估小鼠胰岛β细胞葡萄糖刺激胰岛素分泌的方案。
STAR Protoc. 2021 Aug 9;2(3):100728. doi: 10.1016/j.xpro.2021.100728. eCollection 2021 Sep 17.
4
Preparing Highly Viable Single-Cell Suspensions from Mouse Pancreatic Islets for Single-Cell RNA Sequencing.从小鼠胰岛中制备高活力单细胞悬液用于单细胞 RNA 测序。
STAR Protoc. 2020 Oct 20;1(3):100144. doi: 10.1016/j.xpro.2020.100144. eCollection 2020 Dec 18.
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Engineering the vasculature for islet transplantation.工程化血管用于胰岛移植。
Acta Biomater. 2019 Sep 1;95:131-151. doi: 10.1016/j.actbio.2019.05.051. Epub 2019 May 23.
6
Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice.支架支持的胰岛移植至糖尿病小鼠附睾脂肪垫
J Vis Exp. 2017 Jul 23(125):54995. doi: 10.3791/54995.
7
A bilaminated decellularized scaffold for islet transplantation: Structure, properties and functions in diabetic mice.用于胰岛移植的双层脱细胞支架：结构、特性和在糖尿病小鼠中的功能。
Biomaterials. 2017 Sep;138:80-90. doi: 10.1016/j.biomaterials.2017.05.033. Epub 2017 May 22.