大鼠胰岛肾被膜下隔离与移植治疗糖尿病的方案
Protocol for isolation and transplantation of rat pancreatic islets in the kidney capsule to treat diabetes.
作者信息
Li Dayan, Kong Weijing, Hong Yi, Zhang Wei, Sun Bingbing, Wang Xi, Wang Kai
机构信息
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Clinical Stem Cell Research Center, Peking University Third Hospital, Peking University, Beijing 100191, China.
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Vascular Homeostasis and Remodeling, Clinical Stem Cell Research Center, Peking University Third Hospital, Peking University, Beijing 100191, China.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103486. doi: 10.1016/j.xpro.2024.103486. Epub 2024 Dec 13.
Islet transplantation offers promise for those with type 1 and late-stage type 2 diabetes; however, islet isolation from the pancreas is challenging due to the interference of exocrine tissue. We describe steps for constructing a diabetes model and precise islet isolation by a perfusion-based technique, followed by gradient centrifugation and cultivation in RPMI 1640 for recovery. Transplanted rat islets in diabetic nude mice demonstrated functional recovery, confirming the effectiveness of this approach.
胰岛移植为1型和晚期2型糖尿病患者带来了希望；然而，由于外分泌组织的干扰，从胰腺中分离胰岛具有挑战性。我们描述了构建糖尿病模型和通过基于灌注的技术精确分离胰岛的步骤，随后进行梯度离心，并在RPMI 1640中培养以恢复胰岛功能。将大鼠胰岛移植到糖尿病裸鼠体内显示出功能恢复，证实了该方法的有效性。
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