Key Laboratory of Human Functional Genomics of Jiangsu Province, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
STAR Protoc. 2021 Aug 9;2(3):100728. doi: 10.1016/j.xpro.2021.100728. eCollection 2021 Sep 17.
Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise assessment of β cell function both in vivo and ex vivo. The in vivo assay consists of intravenous tube imbedding surgery and hyperglycemic clamp. The ex vivo assay consists of islet isolation, dynamic perfusion and static immersion. For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).
当感应到高血糖水平时，胰腺胰岛β细胞以双相方式分泌胰岛素。本方案描述了不同阶段胰岛素分泌的评估，以及基础、葡萄糖刺激和总胰岛素分泌能力，从而能够精确评估体内和体外β细胞功能。体内测定包括静脉插管植入手术和高血糖钳夹。体外测定包括胰岛分离、动态灌注和静态浸泡。如需了解本方案使用和执行的详细信息，请参考 Sun 等人（2021 年）。
