Filho David, Guerrero Marcelo, Castro Ricardo, Rafael Diana, Andrade Fernanda, Marican Adolfo, Valdes Oscar, Vargas Esteban, Valenzuela Elisa, Mora Claudia, Durán-Lara Esteban F
Laboratory of Bio & Nano Materials, Drug Delivery and Controlled Release, Department of Microbiology, Faculty of Health Sciences, University of Talca, Talca, Chile; PhD Program in Science, R&D Bioactive Products Department, Chemistry Institute of Natural Resources, University of Talca, Talca, Chile.
Multidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Aplicadas, Facultad de Construcción y Medio Ambiente, Universidad Autónoma de Chile, Talca, Chile.
Colloids Surf B Biointerfaces. 2025 Mar;247:114431. doi: 10.1016/j.colsurfb.2024.114431. Epub 2024 Dec 5.
Hydrogels (HGs) are 3-D polymeric networks with high water content, making them appropriate for biomedical applications such as drug delivery systems. This study examines the impact of agarose in semi-interpenetrating polymer networks (Semi-IPNs) based on poly(acrylic acid) (p(AA)), N, N' Methylenebis(acrylamide) (MBA) and agarose (AGA) on the sustained release of Polymyxin B (PolB). Agarose incorporation improved the mechanical strength, swelling behavior and drug retention capacity of the HG. We synthesized the Semi-IPN HGs via free radical polymerization and characterized their structural and thermal properties using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The features of swelling under physiological conditions were carried out. Additionally, we conducted release kinetics using the three prepared HGs, each of which had a distinct amount of AGA. The findings demonstrated that the Semi-IPN HGs with greater AGA concentrations had drug release profiles that were slower and more sustained, making them perfect for long-term therapeutic uses. We also tested the PolB-loaded HGs' antimicrobial efficacy against Pseudomonas aeruginosa, and they showed sustained antibacterial activity. Using NIH-3T3 fibroblast cells, we verified the HGs' biocompatibility, demonstrating their appropriateness for use in biomedicine. According to these findings, agarose modified Semi-IPN HGs may find application in long-term medication delivery systems that aid in the treatment of infections and promote wound healing.
水凝胶（HGs）是具有高含水量的三维聚合物网络，使其适用于诸如药物递送系统等生物医学应用。本研究考察了基于聚丙烯酸（p(AA)）、N,N'-亚甲基双丙烯酰胺（MBA）和琼脂糖（AGA）的半互穿聚合物网络（Semi-IPNs）中琼脂糖对多粘菌素B（PolB）缓释的影响。琼脂糖的加入改善了水凝胶的机械强度、溶胀行为和药物保留能力。我们通过自由基聚合合成了半互穿聚合物网络水凝胶，并使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、热重分析（TGA）和差示扫描量热法（DSC）对其结构和热性能进行了表征。研究了其在生理条件下的溶胀特性。此外，我们使用三种制备好的水凝胶进行了释放动力学研究，每种水凝胶中AGA的含量都不同。研究结果表明，AGA浓度较高的半互穿聚合物网络水凝胶具有更缓慢、更持久的药物释放曲线，使其非常适合长期治疗用途。我们还测试了负载PolB的水凝胶对铜绿假单胞菌的抗菌效果，它们表现出持续的抗菌活性。我们使用NIH-3T3成纤维细胞验证了水凝胶的生物相容性，证明了它们在生物医学中的适用性。根据这些研究结果，琼脂糖改性的半互穿聚合物网络水凝胶可能在长期药物递送系统中得到应用，有助于治疗感染和促进伤口愈合。
