Transcription factors BnaC09.FUL and BnaC06.WIP2 antagonistically regulate flowering time under long-day conditions in Brassica napus.

Appropriate flowering time in rapeseed (Brassica napus L.) is vital for preventing losses from weather, diseases, and pests. However, the molecular basis of its regulation remains largely unknown. Here, a genome-wide association study identifies BnaC09.FUL, a MADS-box transcription factor, as a promising candidate gene regulating flowering time in B. napus. BnaC09.FUL expression increases sharply in B. napus shoot apices near bolting. BnaC09.FUL overexpression results in early flowering, while BnaFUL mutation causes delayed flowering in B. napus. A zinc finger transcription factor, BnaC06.WIP2, is identified as an interaction partner of BnaC09.FUL, and BnaC06.WIP2 overexpression delays flowering in B. napus, with RNA sequencing revealing its influence on the expression of many flowering-associated genes. We further demonstrate that BnaC06.WIP2 directly represses the expression of BnaA05.SOC1, BnaC03.SOC1, BnaC04.SOC1, BnaC06.FT, BnaA06.LFY, BnaC07.FUL, BnaA08.CAL, and BnaC03.CAL and indirectly inhibits the expression of other flowering time-related genes. Genetic and molecular investigations highlight the antagonistic relationship between BnaC09.FUL and BnaC06.WIP2 in regulating the flowering time in B. napus through direct regulation of the expression of BnaC03.SOC1, BnaA08.CAL, and BnaC03.CAL. Overall, our findings provide a mechanism by which the BnaC09.FUL-BnaC06.WIP2 transcriptional regulatory module controls the flowering time in B. napus.