Min Yuanchang, He Shuangcheng, Wang Xin, Hu Huan, Wei Shihao, Ge Ankang, Jiang Lixi, Yang Saiqi, Guo Yuan, Liu Zijin, Chen Mingxun
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, National Yangling Agricultural Biotechnology & Breeding Center, Shaanxi Key Laboratory of Crop Heterosis, College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China.
Hybrid Rapeseed Research Center of Shaanxi Province, Yangling, Shaanxi 712100, China.
J Genet Genomics. 2025 May;52(5):650-665. doi: 10.1016/j.jgg.2024.12.003. Epub 2024 Dec 12.
Appropriate flowering time in rapeseed (Brassica napus L.) is vital for preventing losses from weather, diseases, and pests. However, the molecular basis of its regulation remains largely unknown. Here, a genome-wide association study identifies BnaC09.FUL, a MADS-box transcription factor, as a promising candidate gene regulating flowering time in B. napus. BnaC09.FUL expression increases sharply in B. napus shoot apices near bolting. BnaC09.FUL overexpression results in early flowering, while BnaFUL mutation causes delayed flowering in B. napus. A zinc finger transcription factor, BnaC06.WIP2, is identified as an interaction partner of BnaC09.FUL, and BnaC06.WIP2 overexpression delays flowering in B. napus, with RNA sequencing revealing its influence on the expression of many flowering-associated genes. We further demonstrate that BnaC06.WIP2 directly represses the expression of BnaA05.SOC1, BnaC03.SOC1, BnaC04.SOC1, BnaC06.FT, BnaA06.LFY, BnaC07.FUL, BnaA08.CAL, and BnaC03.CAL and indirectly inhibits the expression of other flowering time-related genes. Genetic and molecular investigations highlight the antagonistic relationship between BnaC09.FUL and BnaC06.WIP2 in regulating the flowering time in B. napus through direct regulation of the expression of BnaC03.SOC1, BnaA08.CAL, and BnaC03.CAL. Overall, our findings provide a mechanism by which the BnaC09.FUL-BnaC06.WIP2 transcriptional regulatory module controls the flowering time in B. napus.
油菜（Brassica napus L.）适宜的开花时间对于防止因天气、病虫害造成的损失至关重要。然而，其调控的分子基础在很大程度上仍不清楚。在此，一项全基因组关联研究确定了一个MADS-box转录因子BnaC09.FUL，它是调控甘蓝型油菜开花时间的一个很有潜力的候选基因。BnaC09.FUL在甘蓝型油菜接近抽薹的茎尖中表达急剧增加。BnaC09.FUL过表达导致提前开花，而BnaFUL突变导致甘蓝型油菜开花延迟。一个锌指转录因子BnaC06.WIP2被确定为BnaC09.FUL的相互作用伙伴，并且BnaC06.WIP2过表达使甘蓝型油菜开花延迟，RNA测序揭示了它对许多开花相关基因表达的影响。我们进一步证明，BnaC06.WIP2直接抑制BnaA05.SOC1、BnaC03.SOC1、BnaC04.SOC1、BnaC06.FT、BnaA06.LFY、BnaC07.FUL、BnaA08.CAL和BnaC03.CAL的表达，并间接抑制其他开花时间相关基因的表达。遗传和分子研究突出了BnaC09.FUL和BnaC06.WIP2在通过直接调控BnaC03.SOC1、BnaA08.CAL和BnaC03.CAL的表达来调控甘蓝型油菜开花时间方面的拮抗关系。总体而言，我们的研究结果提供了一种机制，即BnaC09.FUL - BnaC06.WIP2转录调控模块控制甘蓝型油菜的开花时间。
