Marpaung David Septian Sumanto, Yap Sinaga Ayu Oshin, Damayanti Damayanti
Department of Biosystems Engineering, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Way Huwi, Kec. Jati Agung, Lampung Selatan, Lampung, 35365, Indonesia.
Department of Biology, Institut Teknologi Sumatera, Jl. Terusan Ryacudu, Way Huwi, Kec. Jati Agung, Lampung Selatan, Lampung, 35365, Indonesia.
Anal Biochem. 2025 Mar;698:115750. doi: 10.1016/j.ab.2024.115750. Epub 2024 Dec 12.
The norovirus (NoV), known for its high contagion rate, is the leading cause of acute gastroenteritis. The development of a NoV vaccine is hindered by significant antigenic variation, lack of suitable models, unknown vaccine protection duration, limited human challenge studies, complex performance patterns, and the absence of a reliable in vitro cultivation system, making prevention, early detection, and control the only effective measures to mitigate outbreaks. This review aims to discuss about several norovirus biosensor for point-of-care analysis. Several innovative biosensors have been developed, including techniques such as electrochemical NoV biosensors, colorimetric NoV biosensors, fluorescence NoV biosensors, CRISPR-based NoV biosensors, and other NoV biosensors. These approaches have detected norovirus in biological samples with high sensitivity and specificity. This biosensing technique holds significant promise, not only in improving the speed and accuracy of diagnostic processes but also in strengthening the global response to norovirus infections, thereby underscoring its pivotal role in public health and disease prevention.
诺如病毒（NoV）以其高传染率而闻名，是急性肠胃炎的主要病因。诺如病毒疫苗的研发受到多种因素阻碍，包括显著的抗原变异、缺乏合适的模型、疫苗保护持续时间未知、人体激发试验有限、复杂的表现模式以及缺乏可靠的体外培养系统，这使得预防、早期检测和控制成为减轻疫情爆发的唯一有效措施。本综述旨在探讨几种用于即时检测分析的诺如病毒生物传感器。已经开发了几种创新的生物传感器，包括电化学诺如病毒生物传感器、比色诺如病毒生物传感器、荧光诺如病毒生物传感器、基于CRISPR的诺如病毒生物传感器以及其他诺如病毒生物传感器等技术。这些方法已在生物样本中高灵敏度和特异性地检测到诺如病毒。这种生物传感技术不仅在提高诊断过程的速度和准确性方面，而且在加强全球对诺如病毒感染的应对方面都具有重大前景，从而凸显了其在公共卫生和疾病预防中的关键作用。
