Zhao Yawen, Li Mingyu, Chen Nuoya, Huang Kaiyang, Wu Xiaofeng, Tan Yanfei, Hu Qinsheng, Luo Rifang, Wang Yunbing
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
Department of Orthopedic Surgery and Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu 610041, China; Department of Orthopedic Surgery, Ya'an People's Hospital, Ya'an 625000, China.
Int J Biol Macromol. 2025 Feb;288:138741. doi: 10.1016/j.ijbiomac.2024.138741. Epub 2024 Dec 12.
Ensuring excellent anticoagulant, anti-inflammatory, and endothelialization properties in vascular stents through coating construction is crucial for their satisfactory performance post-implantation. In this study, we propose a cell-membrane mimetic multifunctional hyaluronic acid (HA)-based coating by combining the aminolyzed methacryloyloxyethyl phosphorylcholine (MPC) copolymer with oxidized hyaluronic acid (Ox-HA) through Schiff base reaction. Compared with traditional anti-fouling design, the composite coating present a stage-specific ability, which can resist the adhesion of blood components, while mediating vascular cell fate with the incorporation of HA. The coating exhibited promoted endothelial cell (EC) growth and inhibited the proliferation of smooth muscle cells (SMCs) by inducing a phenotype change. Besides, the multifunctional HA-based coating can mediate macrophages to a M2 phenotype. The coated stents were implanted in rabbits, and exhibited ideal capabilities in promoting endothelialization and inhibiting inflammation and restenosis in vivo, offering a potential approach to address multifunctionality for vascular implants.
通过涂层构建确保血管支架具有优异的抗凝、抗炎和内皮化性能对于其植入后的良好性能至关重要。在本研究中，我们通过席夫碱反应将氨解的甲基丙烯酰氧基乙基磷酰胆碱（MPC）共聚物与氧化透明质酸（Ox-HA）相结合，提出了一种基于细胞膜模拟的多功能透明质酸（HA）涂层。与传统的防污设计相比，复合涂层具有阶段特异性能力，既能抵抗血液成分的粘附，又能通过掺入HA来介导血管细胞命运。该涂层通过诱导表型变化促进内皮细胞（EC）生长并抑制平滑肌细胞（SMC）增殖。此外，基于多功能HA的涂层可将巨噬细胞介导为M2表型。将涂覆的支架植入兔子体内，在体内表现出促进内皮化、抑制炎症和再狭窄的理想能力，为解决血管植入物的多功能性提供了一种潜在方法。
