Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China; School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:235-43. doi: 10.1016/j.msec.2014.02.008. Epub 2014 Feb 12.
The method of stent implantation is currently considered an effective means of treating atherosclerosis. However, implanting of cardiovascular stent often leads to intimal breakage and hyperplasia. The phenomenon that vascular smooth muscle cells (SMCs) transform from contractile to synthetic phenotype becomes a serious obstacle to intimal recovery. To improve how SMCs transform from a synthetic to contractile phenotype, a technique of coimmobilization was used to form type IV collagen (CoIV) and hyaluronic acid (HA) coating on the widely used stent material, titanium (Ti). In this work, several bio-functional coatings made of CoIV/HA mixtures in different ratios were fabricated on the Ti surface. The quantitative characterization of CoIV showed that introducing HA could enhance the amount of the immobilized CoIV on the alkali activated Ti (TiOH) surface. The immunofluorescence staining results of myosin heavy chain (MHC) and DAPI showed that the coating of CoIV/HA in ratios of 200 μg/ml (M200) and 500 μg/ml (M500) also could promote SMCs expressing more contractile phenotype compared with TiOH/CoIV control samples, while the AO/PI staining results indicated that SMCs on the M200 and M500 samples showed less apoptosis ratio. Thus, we hope that this study can provide more helpful exploration and application for promoting the SMC contractile phenotype on the cardiovascular stents.
支架植入方法目前被认为是治疗动脉粥样硬化的有效手段。然而，心血管支架的植入往往会导致内膜破裂和增生。血管平滑肌细胞（SMC）从收缩型向合成型表型转化的现象成为内膜恢复的严重障碍。为了改善 SMC 从合成型向收缩型的转化，采用共固定化技术在广泛使用的支架材料钛（Ti）上形成 IV 型胶原（CoIV）和透明质酸（HA）涂层。在这项工作中，在 Ti 表面制备了几种不同比例的 CoIV/HA 混合物组成的生物功能涂层。CoIV 的定量表征表明，引入 HA 可以增加碱激活 Ti（TiOH）表面固定 CoIV 的量。肌球蛋白重链（MHC）和 DAPI 的免疫荧光染色结果表明，与 TiOH/CoIV 对照样品相比，CoIV/HA 比例为 200μg/ml（M200）和 500μg/ml（M500）的涂层也能促进 SMC 表达更多的收缩型表型，而 AO/PI 染色结果表明，M200 和 M500 样品上的 SMC 凋亡比例较低。因此，我们希望这项研究能为促进心血管支架上 SMC 的收缩表型提供更多有帮助的探索和应用。
