检测脑膜炎奈瑟菌中ROB β-内酰胺酶基因的快速诊断检测方法。

Rapid diagnostic testing method to detect ROB β-lactamase gene in Neisseria meningitidis.

作者信息

Peifer Andrew, Kidney Anna, Nattanmai Geetha, Wahl Kate, Jose Sherly, Owuor Elizabeth, Randall Linnell, Klingbeil Erin, Musser Kimberlee A, Mitchell Kara

机构信息

Wadsworth Center, New York State Department of Health, PO Box 22002, Albany, NY, 12201-2002, USA.

出版信息

Mol Cell Probes. 2025 Feb;79:102000. doi: 10.1016/j.mcp.2024.102000. Epub 2024 Dec 19.

DOI:10.1016/j.mcp.2024.102000

PMID:39674520

Abstract

bla is the only widely found β-lactamase in Neisseria meningitidis, and its presence is on the rise. To enhance our bacterial meningitis testing procedure, we clinically validated a real-time PCR assay to rapidly detect the bla gene and predict drug resistance in Neisseria meningitidis. A screen of 101 clinical isolates and 37 clinical specimens of blood and cerebrospinal fluid received between January 2018 and June 2024 found 8 isolates and 2 cerebrospinal fluid specimens that were positive for bla.

摘要

bla是在脑膜炎奈瑟菌中唯一广泛发现的β-内酰胺酶，并且其出现呈上升趋势。为了改进我们的细菌性脑膜炎检测程序，我们对一种实时PCR检测法进行了临床验证，以快速检测bla基因并预测脑膜炎奈瑟菌的耐药性。对2018年1月至2024年6月期间收到的101份临床分离株以及37份血液和脑脊液临床标本进行筛查，发现8株分离株和2份脑脊液标本bla呈阳性。