Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Public Health Ontario, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
J Antimicrob Chemother. 2019 Jan 1;74(1):22-28. doi: 10.1093/jac/dky391.
Neisseria meningitidis is rarely penicillin resistant. We describe WGS analysis of a penicillin-resistant N. meningitidis collected from a case of invasive meningococcal disease.
Serogrouping, serotyping and serosubtyping were performed with specific antibodies. β-Lactamase was detected by nitrocefin. MICs were determined by Etest and agar dilution. Sequencing of N. meningitidis genomes was done on the Illumina MiSeq platform and genome data were analysed using the Bacterial Isolate Genome Sequence Database (BIGSdb) on the PubMLST Neisseria website (https://pubmlst.org/neisseria/). Transformation was used to confirm the genetic basis of the penicillin resistance.
An N. meningitidis blood isolate from a female patient in her mid-50s with a painful and septic left shoulder was found to have penicillin MIC values of 3-12 mg/L. The isolate was typed as Y: 14, 19: P1.- and ST3587, and was weakly β-lactamase positive. WGS analysis identified a full-length copy of the β-lactamase gene blaROB-1, which was contained on a 1719 bp insert with a G + C content of 41.7% (versus a G + C content of N. meningitidis of 51.7%), suggesting that the blaROB-1 gene came from a different bacterial species. A GenBank analysis of the blaROB-1 gene insert found 99.77% identity with a DNA segment found in plasmid pB1000' from Haemophilus influenzae. Transformation of a penicillin-susceptible strain with the blaROB-1 gene conferred β-lactamase activity and penicillin resistance.
N. meningitidis serogroup Y, ST3587 can carry and express the blaROB-1 gene, leading to penicillin resistance. It is highly likely that the N. meningitidis isolate acquired the blaROB-1 gene from H. influenzae.
脑膜炎奈瑟菌很少对青霉素产生耐药性。我们描述了从一例侵袭性脑膜炎球菌病患者中分离到的耐青霉素脑膜炎奈瑟菌的 WGS 分析。
使用特异性抗体进行血清群、血清型和血清亚型分析。通过硝噻吩检测β-内酰胺酶。通过 Etest 和琼脂稀释法测定 MIC。使用 Illumina MiSeq 平台对脑膜炎奈瑟菌基因组进行测序，并使用 PubMLST 奈瑟氏菌网站（https://pubmlst.org/neisseria/）上的细菌分离基因组序列数据库（BIGSdb）分析基因组数据。转化用于确认青霉素耐药的遗传基础。
从一名 50 多岁女性患者的血液中分离出的耐青霉素的脑膜炎奈瑟菌，该患者患有疼痛性化脓性左肩。该分离株被鉴定为 Y：14、19：P1.-和 ST3587，并且β-内酰胺酶呈弱阳性。WGS 分析鉴定出完整的 blaROB-1 基因β-内酰胺酶基因，该基因位于 1719bp 的插入片段中，G+C 含量为 41.7%（与脑膜炎奈瑟菌的 G+C 含量为 51.7%相比），表明 blaROB-1 基因来自不同的细菌物种。对 blaROB-1 基因插入片段的 GenBank 分析发现，与流感嗜血杆菌质粒 pB1000'中的一段 DNA 片段具有 99.77%的同一性。将 blaROB-1 基因转化为青霉素敏感株可赋予β-内酰胺酶活性和青霉素耐药性。
脑膜炎奈瑟菌血清群 Y、ST3587 可以携带和表达 blaROB-1 基因，导致青霉素耐药。该脑膜炎奈瑟菌分离株极有可能从流感嗜血杆菌获得 blaROB-1 基因。
