Öztürk Cihat, Akyol Rukiye, Küçükgünay Sadık, Sevim Elif
Department of Medical Microbiology, Faculty of Medicine, Kırşehir Ahi Evran University, Turkey.
Republic of Turkey Ministry of Health, Kırşehir Training and Research Hospital, Turkey.
Adv Clin Exp Med. 2025 Jul;34(7):1175-1181. doi: 10.17219/acem/191058.
The spread of antibiotic-resistance genes among healthcare-associated infections (HAIs) poses serious problems in the treatment of these infections. Recently, these resistance genes have also been shown to be present in integrons.
By focusing on integron-mediated mechanisms of antibiotic resistance, we sought to elucidate the genetic determinants underpinning the development of multidrug resistance in clinical isolates of Acinetobacter baumannii.
In this study, 27 TMP-SXT-resistant A. baumannii isolates were obtained from various clinical samples. Class I and class II integrons were determined using polymerase chain reaction (PCR). Samples were sent for DNA sequence analysis of the integron to a private firm (BMLabosis, Ankara, Turkey). The similarities of the DNA sequences with the associated integron were determined using National Center for Biotechnology Information (NCBI) GenBank.
While all isolates were resistant to TMT-SXT and gentamicin, amikacin and tobramycin resistance rates were detected as 70% and 26%, respectively. Class I and class II integrons were found in 1 strain and 2 isolates, respectively. It was also determined that the dfrA12 gene and the aadA2 gene were found in the class I integrons. It was determined that 2 isolates carrying class II integron had dfrA1 and sat2 genes. Both class I and class II integrons were detected in 1 of these isolates.
Despite the low integron detection in the resistant isolates, with the detection of class I and class II integrons among A. baumannii isolates, it was determined that HAIs could spread very rapidly within the hospital and cause multidrug resistance. This study reveals the need for comprehensive surveillance and molecular characterization of integron-mediated resistance mechanisms to inform effective strategies to combat infections caused by multidrug-resistant (MDR) A. baumannii.
抗生素耐药基因在医疗保健相关感染（HAIs）中的传播给这些感染的治疗带来了严重问题。最近，这些耐药基因也被证明存在于整合子中。
通过关注整合子介导的抗生素耐药机制，我们试图阐明鲍曼不动杆菌临床分离株中多药耐药性发展的遗传决定因素。
在本研究中，从各种临床样本中获得了27株对复方新诺明耐药的鲍曼不动杆菌分离株。使用聚合酶链反应（PCR）检测I类和II类整合子。样本被送往一家私人公司（土耳其安卡拉的BMLabosis）进行整合子的DNA序列分析。使用美国国立生物技术信息中心（NCBI）的GenBank确定DNA序列与相关整合子的相似性。
虽然所有分离株均对复方新诺明和庆大霉素耐药，但阿米卡星和妥布霉素的耐药率分别为70%和26%。分别在1株和2株分离物中发现了I类和II类整合子。还确定在I类整合子中发现了dfrA12基因和aadA2基因。确定携带II类整合子的2株分离物具有dfrA1和sat2基因。在其中1株分离物中同时检测到I类和II类整合子。
尽管在耐药分离株中整合子检测率较低，但在鲍曼不动杆菌分离株中检测到I类和II类整合子，确定HAIs可在医院内迅速传播并导致多药耐药。本研究表明需要对整合子介导的耐药机制进行全面监测和分子特征分析，以为对抗多重耐药（MDR）鲍曼不动杆菌引起的感染的有效策略提供信息。
