Zhao Yubo, Zhang Qing, Wu Bingrong, Zhu Yudong, Ren Hui, Diao Youxiang, Tang Yi, Hu Jingdong
College of Veterinary Medicine, Shandong Agricultural University, No.7 Panhe Street, Tai'an 271017, China.
Institute of Animal Science, Chinese Academy of Agricultural Sciences, China.
Comp Immunol Microbiol Infect Dis. 2025 Jan;116:102290. doi: 10.1016/j.cimid.2024.102290. Epub 2024 Dec 13.
Reticuloendotheliosis virus (REV) is a retrovirus in poultry that can atrophy immune organs and cause immunosuppression and tumor diseases. Our previous results revealed that, in the spleen of SPF chickens infected with REV-SNV, gga-miR-222b-5p expression was upregulated at 7, 14 and 21 dpi, and MAPK10 expression was downregulated, both of which were negatively correlated, with a targeted relationship between the two at 28, 35 and 42 dpi. To understand and analyze the expression patterns of MAPK10 and gga-miR-222b-5p in infected chickens at different times and in different immune organs, qRT-PCR was used to analyze the spleen, bursa of Fabricius and thymus samples of SPF chickens at 7, 14, 21, 28, 35 and 42 dpi. The results revealed that, in the spleen, MAPK10 gene expression was highly significantly downregulated at 7, 14, 21, 35 and 42 dpi, and gga-miR-222b-5p expression was significantly upregulated at six-time points. In the bursa of Fabricius, MAPK10 expression was significantly downregulated at six-time points, and gga-miR-222b-5p expression was upregulated at 7, 21, 28, 35 and 42 dpi. In the thymus, MAPK10 and gga-miR-222b-5p expression was upregulated at six-time points. A negative regulatory relationship was evident in the spleen and bursa of Fabricius but not in the thymus. This study suggested that gga-miR-222b-5p may induce the downregulation of MAPK10 in the spleen and bursa of Fabricius and promote tumor formation.
网状内皮组织增生症病毒（REV）是一种禽类逆转录病毒，可使免疫器官萎缩，导致免疫抑制和肿瘤疾病。我们之前的研究结果显示，在感染REV-SNV的SPF鸡脾脏中，gga-miR-222b-5p在感染后7、14和21天表达上调，而MAPK10表达下调，二者呈负相关，且在感染后28、35和42天存在靶向关系。为了解和分析MAPK10和gga-miR-222b-5p在感染鸡不同时间及不同免疫器官中的表达模式，采用qRT-PCR对感染后7、14、21、28、35和42天的SPF鸡脾脏、法氏囊和胸腺样本进行分析。结果显示，在脾脏中，MAPK10基因在感染后7、14、21、35和42天表达极显著下调，gga-miR-222b-5p在六个时间点均显著上调。在法氏囊中，MAPK10在六个时间点均显著下调，gga-miR-222b-5p在感染后7、21、28、35和42天表达上调。在胸腺中，MAPK10和gga-miR-222b-5p在六个时间点均表达上调。在脾脏和法氏囊中存在明显的负调控关系，但在胸腺中不存在。本研究表明，gga-miR-222b-5p可能诱导脾脏和法氏囊中MAPK10的下调并促进肿瘤形成。
