My Rebecca, Gupte Ameya Pankaj, Bizzotto Edoardo, Frizzarin Martina, Antoniali Paolo, Campanaro Stefano, Favaro Lorenzo
Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, Legnaro 35020, Italy.
Department of Biology, University of Padova, Padova 35131, Italy.
N Biotechnol. 2025 Mar 25;85:63-74. doi: 10.1016/j.nbt.2024.12.004. Epub 2024 Dec 14.
Lignocellulosic biomass holds significant promise as a substrate for bioethanol production, yet the financial viability of lignocellulosic fermentation poses challenges. The pre-treatment step needed for lignocellulosic substrates generates inhibitors that impede Saccharomyces cerevisiae growth, affecting the fermentation process and overall yield. In modern sugarcane-to-ethanol plants, a rapid succession of yeast strains occurs, with dominant strains prevailing. Therefore, yeast strains with both dominance potential and inhibitor tolerance are crucial towards the development of superior strains with industrial fitness. This study adopted a hybrid approach combining biotechnology and bioinformatics to explore a cluster of 20 S. cerevisiae strains, including industrial and oenological strains exhibiting diverse phenotypic features. In-depth genomic analyses focusing on gene copy number variations (CNVs) and single nucleotide polymorphisms (SNPs) were conducted and compared with results from fermentation tests once inoculated in multiple strains kinetics under stressing conditions such as low nitrogen availability and high formic or acetic acid levels. Some strains showed high resistance to biotic stress and acetic acid. Moreover, four out of 20 strains - namely S. cerevisiae YI30, Fp89, Fp90 and CESPLG05 - displayed promising resistance also to formic acid, the most impactful weak acids in pre-treated lignocellulosic biomass. These strains have the potential to be used for the development of superior S. cerevisiae strains tailored for lignocellulosic bioethanol production.
木质纤维素生物质作为生物乙醇生产的底物具有巨大潜力，但木质纤维素发酵的经济可行性面临挑战。木质纤维素底物所需的预处理步骤会产生抑制剂，阻碍酿酒酵母的生长，影响发酵过程和总体产量。在现代甘蔗制乙醇工厂中，酵母菌株会快速连续更替，优势菌株占主导地位。因此，兼具优势潜力和抑制剂耐受性的酵母菌株对于培育具有工业适应性的优良菌株至关重要。本研究采用生物技术与生物信息学相结合的混合方法，对一组20株酿酒酵母菌株进行探索，这些菌株包括具有不同表型特征的工业菌株和酿酒菌株。开展了聚焦于基因拷贝数变异（CNV）和单核苷酸多态性（SNP）的深入基因组分析，并将其与在低氮可用性以及高甲酸或乙酸水平等胁迫条件下接种于多种菌株动力学发酵试验的结果进行比较。一些菌株对生物胁迫和乙酸表现出高抗性。此外，20株菌株中的4株——即酿酒酵母YI30、Fp89、Fp90和CESPLG05——对预处理木质纤维素生物质中影响最大的弱酸甲酸也表现出良好的抗性。这些菌株有潜力用于培育为木质纤维素生物乙醇生产量身定制的优良酿酒酵母菌株。
