Beumer T, Lenssinck H, Pennings A, Haanen C
Cytometry. 1985 Jan;6(1):65-8. doi: 10.1002/cyto.990060112.
Modern flow cytometers offer the possibility to measure more parameters of individual cells than the normally available data acquisition equipment can store. To enlarge the number of parameters that is taken into account while recording data, an analog level discriminator is described that is easy to build and easy to implement on any existing FCM system. An example of gated data acquisition in light scattering measurements on nucleated human bone marrow cells is given. The level discriminator may give a cheap and yet valuable extension of expensive data acquisition equipment.
现代流式细胞仪能够测量单个细胞的更多参数，这超出了通常可用的数据采集设备的存储能力。为了在记录数据时增加所考虑的参数数量，本文描述了一种模拟电平鉴别器，它易于构建且易于在任何现有的流式细胞仪系统上实现。文中给出了一个对有核人类骨髓细胞进行光散射测量时门控数据采集的示例。该电平鉴别器可以为昂贵的数据采集设备提供一种廉价但有价值的扩展。
