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“细胞计量学”数据系统模块——用于流式细胞术数据处理和呈现的现代电子设备。

"Cytomic" data system modules--modern electronic devices for flow cytometric data handling and presentation.

作者信息

Kachel V, Schedler K, Schneider H, Haack L

出版信息

Cytometry. 1984 May;5(3):299-303. doi: 10.1002/cyto.990050313.

Abstract

Flow cytometric investigations can be very complex when several parameters of cells are to be measured and cells must be sorted. On the other hand, many questions may be answered by a single-parameter analysis. The Cytomic modular data system can easily be adapted to such different needs of flow cytometry. It consists of four types of modules which can be used as stand-alone elements or can be combined into a larger data system: CYTOMIC 12, a one-and two-parameter analyzer; CYTOMIC 123, a module mainly provided for on-line three-parameter data uptake and processing; CYTOMIC SORT, a fully software-controlled sort module, where sort windows may be selected by a built-in light pen; and the CYTOMIC LIST list mode module, which is able to collect up to eight cell parameters unclassified on a 1.2-MB floppy disk. Each module built from Z80 microprocessor Eurocards has its own keyboard, display screen, power supply, analog-to-digital pulse height conversion equipment, and an intermodule data communication bus. Each module has a preprogrammed set of functions, which is specially tailored for flow cytometric applications. This kind of data system allows the user to start with a single module for one-and two-parameter analysis. When required, the system may successively be enlarged without losing the stand-alone feature of each module.

摘要

当需要测量细胞的多个参数并且必须对细胞进行分选时,流式细胞术研究可能会非常复杂。另一方面,许多问题可以通过单参数分析来回答。Cytomic模块化数据系统可以轻松适应流式细胞术的此类不同需求。它由四种类型的模块组成,这些模块既可以用作独立元件,也可以组合成一个更大的数据系统:CYTOMIC 12,一种单参数和双参数分析仪;CYTOMIC 123,一个主要用于在线三参数数据采集和处理的模块;CYTOMIC SORT,一个完全由软件控制的分选模块,在这里可以通过内置光笔选择分选窗口;以及CYTOMIC LIST列表模式模块,它能够在一张1.2MB的软盘上收集多达八个未分类的细胞参数。每个由Z80微处理器欧式卡构建的模块都有自己的键盘、显示屏、电源、模数脉冲高度转换设备以及模块间数据通信总线。每个模块都有一组预编程的功能,这些功能是专门为流式细胞术应用量身定制的。这种数据系统允许用户从用于单参数和双参数分析的单个模块开始。如有需要,该系统可以逐步扩展,而不会失去每个模块的独立特性。

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