Fu Qiqi, Yang Jiali, Jiang Hui, Ren Yi, Huo Longfei, Liu Muqing
School of Information Science and Technology, Fudan University, Shanghai, China.
Academy for Engineering and Technology, Fudan University, Shanghai, China.
J Biophotonics. 2025 Feb;18(2):e202400398. doi: 10.1002/jbio.202400398. Epub 2024 Dec 15.
Increasing evidence has underscored the pivotal role of red photobiomodulation (R-PBM) in analgesic and anti-inflammatory processes; nonetheless, research concerning the effects of pulsed wave on primary dysmenorrhea (PD) remains sparse. This study found that pulsed R-PBM significantly diminished pain responses and levels of PGF/PGE, mitigated uterine swelling, augmented antioxidant capacity, and lowered MDA concentrations, which outperformed continuous wave at the same average irradiance. Furthermore, PW treatment substantially reduced ROS levels and enhanced cell viability in PGF induced HUSM cells. NOS levels, especially iNOS, were markedly diminished in the uteri of PD mice, accompanied by significant alterations in inflammation-related genes (Jun, Fos, IL1rn, IL17b) and protein levels, along with pronounced downregulation of calcium ion concentrations after pulsed R-PBM intervention. These findings indicated that pulsed R-PBM may mitigate pain by modulating ROS and NO/NOS, mediated oxidative stress and inflammatory responses. Consequently, pulsed R-PBM emerges as a promising therapeutic strategy for PD.
越来越多的证据强调了红光生物调制（R-PBM）在镇痛和抗炎过程中的关键作用；然而，关于脉冲波对原发性痛经（PD）影响的研究仍然很少。本研究发现，脉冲R-PBM显著降低了疼痛反应和PGF/PGE水平，减轻了子宫肿胀，增强了抗氧化能力，并降低了MDA浓度，在相同平均辐照度下，其效果优于连续波。此外，脉冲波治疗显著降低了PGF诱导的人子宫平滑肌细胞（HUSM）中的ROS水平，并提高了细胞活力。在PD小鼠的子宫中，NOS水平，尤其是诱导型一氧化氮合酶（iNOS）水平显著降低，同时炎症相关基因（Jun、Fos、IL1rn、IL17b）和蛋白质水平发生显著变化，并且在脉冲R-PBM干预后钙离子浓度明显下调。这些发现表明，脉冲R-PBM可能通过调节ROS和NO/NOS、介导氧化应激和炎症反应来减轻疼痛。因此，脉冲R-PBM成为一种有前景的PD治疗策略。
