Fu Qiqi, Yang Jiali, Jiang Hui, Lin Shangfei, Qin Haokuan, Zhao Jie, Wang Yanqing, Liu Muqing
School of Information Science and Technology, Fudan University, Shanghai, China.
Academy for Engineering and Technology, Fudan University, Shanghai, China.
J Biophotonics. 2024 May;17(5):e202300448. doi: 10.1002/jbio.202300448. Epub 2024 Feb 13.
Photobiomodulation (PBM) has attracted widespread attention in suppressing various pain and inflammation. Primary dysmenorrhea (PD) primarily occurs in adolescents and adult females, and the limited effectiveness and side effects of conventional treatments have highlighted the urgent need to develop and identify new adjunct therapeutic strategies. In this work, the results of pain and PGs demonstrated that 850 nm, 630 nm, and 460 nm all exhibited pain inhibition, decreased PGF and upregulated PGE, while 630 nm PBM has better effectiveness. Then to explore the underlying biological mechanisms of red light PBM on PD, we irradiated prostaglandin-F induced HUSM cells and found that low-level irradiance can restore intracellular calcium ion, ROS, ATP, and MMP levels to normal levels. And, red light enhanced cell viability and promoted cell proliferation for normal HUSM cells. Therefore, this study proposes that red light PBM may be a promising approach for the future clinical treatment of PD.
光生物调节作用（PBM）在抑制各种疼痛和炎症方面已引起广泛关注。原发性痛经（PD）主要发生在青少年和成年女性中，传统治疗方法的有限疗效和副作用凸显了开发和确定新的辅助治疗策略的迫切需求。在这项研究中，疼痛和前列腺素（PGs）的结果表明，850纳米、630纳米和460纳米均表现出疼痛抑制作用，降低了前列腺素F（PGF）并上调了前列腺素E（PGE），而630纳米的PBM具有更好的疗效。然后，为了探究红光PBM对PD的潜在生物学机制，我们用前列腺素F诱导人子宫平滑肌（HUSM）细胞进行照射，发现低水平辐照度可将细胞内钙离子、活性氧（ROS）、三磷酸腺苷（ATP）和线粒体膜电位（MMP）水平恢复到正常水平。并且，红光增强了正常HUSM细胞的活力并促进了细胞增殖。因此，本研究提出红光PBM可能是未来PD临床治疗的一种有前景的方法。
