Oishi S
Environ Res. 1985 Feb;36(1):160-9. doi: 10.1016/0013-9351(85)90014-3.
Previous studies have shown that di(2-ethylhexyl) phthalate (DEHP) results in atrophy of testes and accessory sex organs accompanied by a decreased testicular concentration of zinc in rats. An experiment was performed to determine whether those changes in rat testes are reversible changes. Young Wistar male rats were administered 2.0 g/kg of DEHP for 14 days. At that time, one-half of the rats were killed for determination of zinc and testosterone concentrations in the testis, liver, and serum, and organ weights. The remaining rats were maintained for additional 45 days without further DEHP administration. Testicular weight of rats administered DEHP over the 14-day period was significantly less than that of the control animals, and testicular testosterone content and zinc concentrations were less than those of the control animals. At the end of the 45-day recovery phase, serum testosterone concentration returned to the control level, but testicular zinc concentration and testosterone content were still lower than those of the control animals. In addition, testicular weight of DEHP-treated rats was lower than that of the control animals and histologically, only a small number of seminiferous tubules showed spermatogenesis. These results indicate that, morphologically, DEHP-induced testicular atrophy appears to be of limited reversibility.
先前的研究表明，邻苯二甲酸二（2-乙基己基）酯（DEHP）会导致大鼠睾丸和附属生殖器官萎缩，同时睾丸中的锌浓度降低。进行了一项实验，以确定大鼠睾丸的这些变化是否为可逆变化。将年轻的Wistar雄性大鼠给予2.0 g/kg的DEHP，持续14天。此时，将一半的大鼠处死，以测定睾丸、肝脏和血清中的锌和睾酮浓度以及器官重量。其余的大鼠在不进一步给予DEHP的情况下再维持45天。在14天期间给予DEHP的大鼠的睾丸重量明显低于对照动物，睾丸睾酮含量和锌浓度也低于对照动物。在45天的恢复期结束时，血清睾酮浓度恢复到对照水平，但睾丸锌浓度和睾酮含量仍低于对照动物。此外，DEHP处理的大鼠的睾丸重量低于对照动物，并且在组织学上，只有少数生精小管显示有精子发生。这些结果表明，在形态学上，DEHP诱导的睾丸萎缩似乎具有有限的可逆性。
