Kim Ji-Young, Shin Geun-Seup, An Mi-Jin, Lee Hyun-Min, Jo Ah-Ra, Park Yuna, Kim Jinho, Hwangbo Yujeong, Kim Chul-Hong, Kim Jung-Woong
Department of Life Science, Chung-Ang University, Heukseok-ro 84, Dongjak-gu, Seoul 06974, South Korea.
Toxicol Res (Camb). 2024 Dec 1;13(6):tfae200. doi: 10.1093/toxres/tfae200. eCollection 2024 Dec.
Bisphenols are prevalent in food, plastics, consumer goods, and industrial products. Bisphenol A (BPA) and its substitutes, bisphenol F (BPF) and bisphenol S (BPS), are known to act as estrogen mimics, leading to reproductive disorders, disruptions in fat metabolism, and abnormalities in brain development.
Despite numerous studies exploring the adverse effects of bisphenols both and , the molecular mechanisms by which these compounds affect lung cells remain poorly understood. This study aims to compare the effects of BPA, BPF, and BPS on the physiological behavior of human nonsmall cell lung cancer (NSCLC) cells.
Human non-small cell lung cancer (NSCLC) H1299 cells were treated with various concentration of BPA, BPF and BPS during different exposure time. Cellular physiology for viability and cell cycle was assessed by the staining with apoptotic cell makers such as active Caspase-3 and cyclins antibodies. Toxicological effect was quantitatively counted by using flow-cytometry analysis.
Our findings indicate that BPA induces apoptosis by increasing active Caspase-3 levels in H1299 cells, whereas BPF and BPS do not promote late apoptosis. Additionally, BPA was found to upregulate cyclin B1, causing cell cycle arrest at the G0/G1 phase and leading to apoptotic cell death through Caspase-3 activation. Conclusion: These results demonstrate that BPA, BPF, and BPS differentially impact cell viability, cell cycle progression, and cell death in human NSCLC cells.
双酚类物质广泛存在于食品、塑料、消费品和工业产品中。已知双酚A（BPA）及其替代品双酚F（BPF）和双酚S（BPS）具有雌激素模拟作用，可导致生殖紊乱、脂肪代谢失调和大脑发育异常。
尽管已有大量研究探讨了双酚类物质的不良影响，但这些化合物影响肺细胞的分子机制仍知之甚少。本研究旨在比较BPA、BPF和BPS对人非小细胞肺癌（NSCLC）细胞生理行为的影响。
在不同暴露时间用不同浓度的BPA、BPF和BPS处理人非小细胞肺癌（NSCLC）H1299细胞。通过用凋亡细胞标记物如活性半胱天冬酶-3和细胞周期蛋白抗体染色来评估细胞活力和细胞周期的细胞生理学。使用流式细胞术分析定量计算毒理学效应。
我们的研究结果表明，BPA通过增加H1299细胞中活性半胱天冬酶-3的水平诱导凋亡，而BPF和BPS不促进晚期凋亡。此外，发现BPA上调细胞周期蛋白B1，导致细胞周期停滞在G0/G1期，并通过半胱天冬酶-3激活导致凋亡细胞死亡。结论：这些结果表明，BPA、BPF和BPS对人NSCLC细胞的细胞活力、细胞周期进程和细胞死亡有不同影响。
