通过色谱聚焦法分离中间丝蛋白波形蛋白。
Isolation of the intermediate filament protein vimentin by chromatofocusing.
作者信息
Bloemendal H, Willemsen M, Groenewoud G, Oomen P
出版信息
FEBS Lett. 1985 Jan 28;180(2):181-4. doi: 10.1016/0014-5793(85)81067-x.
PMID:3967763
Abstract
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22 degrees C is 4.9. Electrophoretic mobility on one- and two-dimensional polyacrylamide gels, solubility in 6 M urea and amino acid composition were used for identification.
摘要
本文描述了一种从眼晶状体组织中分离中等大小的丝状蛋白波形蛋白的新颖、简单且相对快速的方法。仅使用层析聚焦作为纯化步骤。该蛋白在6M尿素中、22℃时的表观等电点为4.9。通过一维和二维聚丙烯酰胺凝胶电泳迁移率、在6M尿素中的溶解度以及氨基酸组成进行鉴定。
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