Li Yanxi, He Wenting, Liu Yueyue, Mei Chendi, Wang Hai, Song Xuebin
College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao, Shandong, China.
PeerJ. 2024 Dec 12;12:e18620. doi: 10.7717/peerj.18620. eCollection 2024.
(× Ramat.) is a particularly important autumn perennial flower for potted plant, flower bed and border, and cut flower with high ornamental value. However, abiotic stress can affect the ornamental quality of . NAC (NAM, ATAF1-2, and CUC2) transcription factors (TFs) play an important role in regulating plant growth and development, as well as responding to abiotic stresses.
In this study, the ( BEARSKIN gene) was isolated from the model plant . And analyze the function of the gene through bioinformatics, subcellular localization and overexpression.
Bioinformatics analysis showed that the gene was a member of the NAC TFs family, with a CDS (coding sequence) length of 1,080 bp and encoding 359 amino acids. The subcellular localization results found that the gene was located in the nucleus and cell membrane. Furthermore, the transgenic results in showed that the gene significantly reduces plant height while improving salt and low temperature tolerance. Observation of paraffin sections of stems also revealed that the secondary cell wall of overexpressing stems was significantly thicker than that of wild-type. The above results indicate that the gene may play an important role in regulating plant resistance to abiotic stress. This study will provide new insights for molecular breeding of resistant chrysanthemums in the future.
（×拉马特。）是一种特别重要的秋季多年生花卉，适用于盆栽植物、花坛和花境，以及具有高观赏价值的切花。然而，非生物胁迫会影响其观赏品质。NAC（NAM、ATAF1 - 2和CUC2）转录因子在调节植物生长发育以及应对非生物胁迫方面发挥着重要作用。
在本研究中，从模式植物中分离出（BEARSKIN基因）。并通过生物信息学、亚细胞定位和过表达分析该基因的功能。
生物信息学分析表明，该基因是NAC转录因子家族的成员，编码序列（CDS）长度为1080 bp，编码359个氨基酸。亚细胞定位结果发现该基因位于细胞核和细胞膜中。此外，在中的转基因结果表明，该基因显著降低了株高，同时提高了耐盐性和耐低温性。对茎的石蜡切片观察还发现，过表达茎的次生细胞壁明显比野生型厚。上述结果表明，该基因可能在调节植物对非生物胁迫的抗性中发挥重要作用。本研究将为未来抗性菊花的分子育种提供新的见解。
