Lim Dong-Kwon, Kumar Panangattukara Prabhakaran Praveen
KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
Department of Integrative Energy Engineering, College of Engineering, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
Nanophotonics. 2024 Mar 6;13(9):1521-1534. doi: 10.1515/nanoph-2023-0362. eCollection 2024 Apr.
Raman scattering can provide information on molecular fingerprints, which have been widely applied in various fields of material science and nanobiotechnology. Notably, low interference with water molecules in obtaining the Raman spectra between 500 and 2000 cm made it a powerful spectroscopic tool in biology, such as imaging and signaling for a living cell. To be a robust tool for cell biology, the performance of obtaining molecular-specific information with high sensitivity, high resolution in real time, and without inducing cell damage is strongly required. The conventional fluorescence-based method has been suffered from the rapid photobleaching of organic fluorophores and the lack of molecular information. In contrast, Raman scattering is a promising spectroscopic tool to acquire cellular information, and the extremely low signal intensity of Raman scattering could be amplified by incorporating the plasmonic nanomaterials. Along with the fundamental research focus on surface-enhanced Raman scattering (SERS), the practical approaches of SERS for cellular imaging as a new tool for drug screening and monitoring cellular signals have been extensively explored based on new optical setups and new designing strategies for the nanostructures. Diverse nanostructure and surface chemistry for targeting or sensing have been played pivotal roles in acquiring cellular information and high resolution cell imaging. In this regard, this review focused on the recent advances of SERS-based technologies for a live cell imaging investigated such as potential drug screening, signaling for chemicals or biomolecules in cell, sensing, and high spatiotemporal resolution.
拉曼散射能够提供有关分子指纹的信息，这些信息已在材料科学和纳米生物技术的各个领域中得到广泛应用。值得注意的是，在获取500至2000厘米之间的拉曼光谱时，它对水分子的干扰较小，这使其成为生物学中一种强大的光谱工具，例如用于活细胞的成像和信号传导。要成为细胞生物学的可靠工具，强烈需要具备以高灵敏度、高分辨率实时获取分子特异性信息且不引起细胞损伤的性能。传统的基于荧光的方法一直受到有机荧光团快速光漂白和缺乏分子信息的困扰。相比之下，拉曼散射是一种有前景的获取细胞信息的光谱工具，通过结合等离子体纳米材料可以放大拉曼散射极低的信号强度。随着对表面增强拉曼散射（SERS）基础研究的关注，基于新的光学装置和纳米结构的新设计策略，作为药物筛选和监测细胞信号的新工具，SERS在细胞成像方面的实际应用方法已得到广泛探索。用于靶向或传感的各种纳米结构和表面化学在获取细胞信息和高分辨率细胞成像中发挥了关键作用。在这方面，本综述重点关注基于SERS的活细胞成像技术的最新进展，研究内容包括潜在的药物筛选、细胞内化学物质或生物分子的信号传导、传感以及高时空分辨率等。
