Department of Mechanical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University, School of Medicine, Baltimore, Maryland, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2022 Jul;14(4):e1802. doi: 10.1002/wnan.1802. Epub 2022 May 5.
Continuous long-term intracellular imaging and multiplexed monitoring of biomolecular changes associated with key cellular processes remains a challenge for the scientific community. Recently, surface-enhanced Raman scattering (SERS) has been demonstrated as a powerful spectroscopic tool in the field of biology owing to its significant advantages. Some of these include the ability to provide molecule-specific information with exquisite sensitivity, working with small volumes of precious samples, real-time monitoring, and optimal optical contrast. More importantly, the availability of a large number of novel Raman reporters with narrower full width at half maximum (FWHM) of spectral peaks/vibrational modes than conventional fluorophores has created a versatile palette of SERS-based probes that allow targeted multiplex sensing surpassing the detection sensitivity of even fluorescent probes. Due to its nondestructive nature, its applicability has been recognized for biological sensing, molecular imaging, and dynamic monitoring of complex intracellular processes. We critically discuss recent developments in this area with a focus on different applications where SERS has been used for obtaining information that remains elusive for conventional imaging methods. Current reports indicate that SERS has made significant inroads in the field of biology and has the potential to be used for in vivo human applications. This article is categorized under: Diagnostic Tools > In Vitro Nanoparticle-Based Sensing Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Diagnostic Tools > Biosensing Diagnostic Tools > In Vivo Nanodiagnostics and Imaging.
持续的长期细胞内成像和与关键细胞过程相关的生物分子变化的多路复用监测仍然是科学界的一个挑战。最近，由于其显著的优势，表面增强拉曼散射（SERS）已被证明是生物学领域的一种强大的光谱工具。其中一些优点包括能够提供具有极高灵敏度的分子特异性信息、处理小体积的珍贵样品、实时监测和最佳的光学对比度。更重要的是，大量新型拉曼报告分子的可用性，其光谱峰/振动模式的半峰全宽（FWHM）比传统荧光团更窄，创造了基于 SERS 的探针的多功能调色板，允许靶向多路复用传感，超过甚至荧光探针的检测灵敏度。由于其非破坏性性质，其适用性已被认可用于生物传感、分子成像和复杂细胞内过程的动态监测。我们批判性地讨论了该领域的最新进展，重点介绍了 SERS 用于获取传统成像方法难以获得的信息的不同应用。目前的报告表明，SERS 在生物学领域取得了重大进展，并有潜力用于体内人类应用。本文属于以下类别：诊断工具 > 体外基于纳米颗粒的传感 纳米技术在生物学中的应用 > 生物学中的纳米级系统 诊断工具 > 生物传感 诊断工具 > 体内纳米诊断和成像。
