Two Strains of Endophytic Carrying Antibiotic-Biosynthetic Genes Show Antibacterial and Antibiofilm Activities Against Methicillin-Resistant (MRSA).

UNLABELLED

Methicillin-resistant (MRSA) is considered a priority pathogen causing high mortality that requires effective control measures. This study aimed to detect the presence of antibiotic-biosynthetic genes and to evaluate the anti-MRSA activity of two strains of endophytic isolated from . PCR-based screening showed that strains, such as DJ4 and DJ9 possessed six antibiotic-biosynthetic genes, namely , and According to the preliminary test conducted using disc-diffusion assay, metabolite extracts from these strains have anti-MRSA activity with clear zone diameters of 13.00 ± 0.82 mm, and 17.33 ± 0.47 mm, respectively. Extract from DJ9 strain was more active to MRSA, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 62.50 µg/mL and 250 µg/mL, respectively. Furthermore, a bactericidal effect was observed, as evidenced by MBC/MIC ratio of four. Both DJ9 and DJ4 extracts showed a dose-dependent inhibitory effect on MRSA biofilm formation. Furthermore, a maximum inhibition percentage of 60.12 ± 2.5% was shown by DJ9 extract in two-fold MIC. The corresponding extract disrupted MRSA mature biofilms most effectively at 55.74 ± 1.4%. In conclusion, crude extract, particularly the DJ9 strain had significant potential in inhibiting MRSA cell growth, MRSA biofilm formation, and disrupting MRSA mature biofilm in vitro.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12088-024-01262-1.