Tóthová Csilla, Sesztáková Edina, Galiková Blanka, Glembová Veronika, Oršuľaková Veronika, Nagy Oskar
Clinic of Ruminants, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice 041 81, Slovakia.
Clinic of Birds, Exotic and Free Living Animals, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, Košice 041 81, Slovakia.
Vet Med Int. 2024 Dec 7;2024:4135744. doi: 10.1155/vmi/4135744. eCollection 2024.
The objective of this study was to characterize the distribution of serum proteins in laying hens reared under cage-free open farm conditions and to evaluate the changes in the concentrations of serum protein fractions throughout the 1-year laying period. Ten Dekalb White white-egg-laying hens were blood sampled from the wing vein at 17, 22, 29, 38, 50, 60, and 70 weeks of age. Egg laying was observed at 18 weeks of age. The average daily egg production in the initial phase of laying (week 22) was 6.3, reaching the maximum (8.5 eggs) in week 38. After a slight decrease, from week 60, the egg production increased repeatedly and stayed relatively constant till the end of the study (8.1-8.3 eggs). The serum samples were analyzed for the concentrations of total proteins and the electrophoretic pattern of serum proteins. Five protein fractions were identified on the serum protein electrophoretogram of the hens, including albumin, -, -, -, and -globulins. The size and shape of the fractions differed significantly according to the stage of the laying period. Significant changes were detected in the ratio of all individual protein fractions as well as in the concentrations of total proteins and protein fractions. The concentrations of total proteins, albumin, -, -, and -globulins decreased significantly after the beginning of egg production compared to values recorded before laying ( < 0.05) and started to increase after reaching the maximum egg production in week 38. The A/G ratios were higher before and at the beginning of the laying period, and the lowest values were obtained in weeks with the highest egg production. These findings suggest that the beginning of egg production is the most critical period in the laying cycle of hens reared under alternative farming conditions characterized by the most marked alterations in the serum protein profile.
本研究的目的是描述在无笼开放式农场条件下饲养的蛋鸡血清蛋白的分布情况，并评估在1年的产蛋期内血清蛋白组分浓度的变化。从10只迪卡白蛋鸡的翅静脉采集血液样本，采样时间分别为17、22、29、38、50、60和70周龄。在18周龄时观察产蛋情况。产蛋初期（第22周）的平均日产蛋量为6.3枚，在第38周达到最高值（8.5枚）。在略有下降后，从第60周开始，产蛋量反复增加，并在研究结束时保持相对稳定（8.1 - 8.3枚）。对血清样本进行总蛋白浓度分析和血清蛋白电泳图谱分析。在母鸡的血清蛋白电泳图谱上鉴定出5种蛋白组分，包括白蛋白、α1 -、α2 -、β -和γ -球蛋白。这些组分的大小和形状根据产蛋期的阶段有显著差异。所有单个蛋白组分的比例以及总蛋白和蛋白组分的浓度均检测到显著变化。与产蛋前记录的值相比，产蛋开始后总蛋白、白蛋白、α1 -、α2 -和β -球蛋白的浓度显著降低（P < 0.05），并在第38周达到最高产蛋量后开始增加。白蛋白/球蛋白（A/G）比值在产蛋期之前和开始时较高，在产蛋量最高的几周获得最低值。这些发现表明产蛋开始是在替代养殖条件下饲养的母鸡产蛋周期中最关键的时期，其血清蛋白谱变化最为明显。
