Cray Carolyn, King Ed, Rodriguez Marilyn, Decker Lilli S, Arheart Kristopher L
Division of Comparative Pathology, Department of Pathology, University of Miami Miller School of Medicine, PO Box 016960 (R-46), Miami, FL 33101, USA.
J Avian Med Surg. 2011 Jun;25(2):102-10. doi: 10.1647/2010-019.1.
Previous studies have defined the presence of 6 protein fractions in plasma from many psittaciform species. Additionally, extensive reference intervals have been published for many of these species with the Beckman Paragon electrophoresis system, which had been commonly used in clinical laboratories to analyze the protein fractions of avian plasma. In mid-2009, Beckman discontinued the Paragon product line, leaving 2 primary alternative systems: Helena and Sebia. To compare electrophoresis results from the 3 commercial protein electrophoresis systems, specimens from 40 African grey parrots (Psittacus erithacus) were analyzed with the electrophoresis systems from Beckman, Helena, and Sebia. Marked differences in fraction migration were found between the Beckman/Helena and Sebia systems, which manifested as a large decrease in prealbumin and an increase in alpha1 globulins in the latter system. Both proportional and constant errors were observed among the fraction quantitation data of both the Helena and Sebia systems compared with the Beckman system. Based on Bland-Altman plot data and imprecision studies, the Helena system appears more similar with the Beckman system, although neither the Helena nor the Sebia systems are identical to the Beckman system. Because of the differences in electrophoresis methods, clinicians should be careful to consistently use particular clinical laboratories. For best application, reference intervals should be established based on both species and electrophoresis system.
先前的研究已确定许多鹦鹉科物种的血浆中存在6种蛋白质组分。此外，对于其中许多物种，已经使用临床实验室常用的贝克曼Paragon电泳系统公布了广泛的参考区间，该系统用于分析鸟类血浆的蛋白质组分。2009年年中，贝克曼停产了Paragon产品线，剩下2种主要的替代系统：海伦娜（Helena）和赛比亚（Sebia）。为了比较3种商用蛋白质电泳系统的电泳结果，用贝克曼、海伦娜和赛比亚的电泳系统对40只非洲灰鹦鹉（Psittacus erithacus）的样本进行了分析。在贝克曼/海伦娜系统和赛比亚系统之间发现了组分迁移的显著差异，表现为后一种系统中前白蛋白大幅减少而α1球蛋白增加。与贝克曼系统相比，海伦娜系统和赛比亚系统的组分定量数据均观察到比例误差和恒定误差。基于布兰德-奥特曼图数据和不精密度研究，海伦娜系统似乎与贝克曼系统更相似，尽管海伦娜系统和赛比亚系统都与贝克曼系统不完全相同。由于电泳方法的差异，临床医生应谨慎始终使用特定的临床实验室。为了获得最佳应用效果，应根据物种和电泳系统建立参考区间。
