Corbi A L, Carreira J
Int Arch Allergy Appl Immunol. 1985;76(2):156-61. doi: 10.1159/000233683.
Parietaria judaica pollen extract was separated by preparative isoelectric focusing and showed two allergenic components (A1 and A2) heterogeneously distributed within the pH gradient (pH 3-10). By fused-rocket immunoelectrophoresis, A1 was detected from pH 3 to 10, whereas A2 was only found at pH values below 8. Both components, A1 and A2, showed IgE-binding ability throughout their pH ranges, as determined by crossed-radio immunoelectrophoresis. In addition, all the preparative isoelectric focusing fractions contained the main allergenic polypeptide of P. judaica (Pj10) when analyzed by SDS-PAGE. This polypeptide was shown to be a glycopeptide or to be bound to a glycoprotein with alpha-D-mannopyranoside or alpha-D-glycopyranoside residues. Implication of charged sugar residues in the wide distribution of P. judaica allergens in a pH gradient is discussed.
墙草花粉提取物通过制备性等电聚焦进行分离，结果显示在pH梯度（pH 3 - 10）内有两种变应原成分（A1和A2）呈不均匀分布。通过火箭免疫电泳法，在pH 3至10范围内检测到A1，而A2仅在pH值低于8时被发现。通过交叉放射免疫电泳测定，A1和A2两种成分在其整个pH范围内均显示出IgE结合能力。此外，当通过SDS - PAGE分析时，所有制备性等电聚焦级分均含有墙草的主要变应原多肽（Pj10）。该多肽显示为糖肽或与带有α - D - 甘露吡喃糖苷或α - D - 葡糖吡喃糖苷残基的糖蛋白结合。文中讨论了带电荷的糖残基在墙草变应原在pH梯度中广泛分布中的作用。
