Corbi A L, Carreira J
Int Arch Allergy Appl Immunol. 1984;74(4):318-23. doi: 10.1159/000233567.
Allergens of Parietaria judaica pollen extract have been identified and characterized biochemically. Two main allergenic components, A1 and A2, have been found by crossed-radioimmunoelectrophoresis and demonstrated to be spread in a wide range of pH. Immunoblotting studies revealed that at least eight SDS-denatured polypeptides show IgE-binding activity. The one exhibiting the highest allergenic activity, named Pj10 (MW 10,000 daltons) was found in all the fractions when the pollen extract was fractionated by chromatofocusing. Bidimensional electrophoretic analysis suggested that Pj10 either can form homopolymeric proteins of different molecular weights or can be associated to a number of proteins by disulfide bridges. Furthermore, Pj10 is the main molecular structure with IgE-binding activity in the two allergenic components A1 and A2 defined by immunological criteria.
墙草花粉提取物的过敏原已被鉴定并进行了生化特性分析。通过交叉放射免疫电泳发现了两种主要的过敏原成分A1和A2，并证明它们在很宽的pH范围内分布。免疫印迹研究表明，至少有八种SDS变性多肽具有IgE结合活性。当通过色谱聚焦对花粉提取物进行分级分离时，在所有级分中都发现了具有最高过敏原活性的一种，命名为Pj10（分子量10,000道尔顿）。双向电泳分析表明，Pj10要么可以形成不同分子量的同聚蛋白，要么可以通过二硫键与多种蛋白质结合。此外，Pj10是通过免疫学标准定义的两种过敏原成分A1和A2中具有IgE结合活性的主要分子结构。
