Wang Linlin, Tang Ruitong, Wang Wentao, Bu Lingguang, Sun Jingle, Fu Yurong, Li Meng, Yi Zhengjun
School of Medical Laboratory, Shandong Second Medical University, Weifang 261053, Shandong Province, China.
Department of Medical Microbiology, School of Basic Medicine, Shandong Second Medical University, Weifang, China.
Anal Methods. 2025 Jan 23;17(4):652-664. doi: 10.1039/d4ay01106f.
Coronavirus disease 2019 (COVID-19), an infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread globally, posing a significant threat to human health. Rapid and accurate detection of infectious disease pathogens is of crucial practical significance for early screening, timely intervention, and outbreak prevention. However, conventional diagnostic methods are increasingly unable to meet clinical demands. Recently developed isothermal analysis methods offer mild reaction conditions and reduce dependence on specialized instruments. These convenient, fast, and reliable methods show great promise for diagnosing infectious pathogens, especially for on-site detection in areas without laboratories or with limited resources. Among them, loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), which integrate various widely used detection techniques, stand out as rapidly advancing and relatively mature isothermal nucleic acid amplification technologies. This review outlines several representative isothermal amplification technologies and associated detection methods. We summarize the latest advancements in LAMP and RPA technologies for the rapid detection of SARS-CoV-2 and discuss the future prospects of isothermal amplification in diversified testing.
2019冠状病毒病（COVID-19）是由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的一种传染病，已在全球范围内传播，对人类健康构成重大威胁。快速准确地检测传染病病原体对于早期筛查、及时干预和疫情防控具有至关重要的现实意义。然而，传统的诊断方法越来越无法满足临床需求。最近开发的等温分析方法反应条件温和，减少了对专用仪器的依赖。这些方便、快速且可靠的方法在诊断传染病病原体方面显示出巨大潜力，特别是对于在没有实验室或资源有限的地区进行现场检测。其中，整合了各种广泛使用的检测技术的环介导等温扩增（LAMP）和重组酶聚合酶扩增（RPA），作为快速发展且相对成熟的等温核酸扩增技术脱颖而出。本文综述了几种具有代表性的等温扩增技术及相关检测方法。我们总结了LAMP和RPA技术在快速检测SARS-CoV-2方面的最新进展，并探讨了等温扩增在多样化检测中的未来前景。
