Manzoor Natasha, Samad Noreen, Bhatti Sheraz Ahmed, Irfan Ali, Ahmad Sadaf, Shazly Gamal A, Bin Jardan Yousef A
Department of Biochemistry, Faculty of Science, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Department of Pathobiology, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Dec 16. doi: 10.1007/s00210-024-03687-3.
This study investigates the impact of a high-fat-rich diet (HFRD) on behavioral, biochemical, neurochemical, and histopathological studies using the hypothalamus of rats following niacin (NCN) administration. The rats were divided into HFRD and normal diet (ND)-fed groups and administered selected doses of NCN, i.e., 25 mg/mL/kg (low dose) and 50 mg/mL/kg (high dose), for 8 weeks. The grouping of male rats (n = 8) was as follows: (i) Vehicle (Veh) + ND; (ii) ND + NCN (low dose); (iii) ND + NCN (high dose); (iv) Veh + HFRD; (v) HFRD + NCN (low dose); and (vi) HFRD + NCN (high dose). Behavioral tests assessed depression-like symptoms and spatial memory; after that, the hypothalamus was isolated for various analyses of sacrificed animals. NCN at both doses decreased food intake and growth rate in both diet groups and demonstrated antidepressant and memory-enhancing effects. HFRD-induced oxido-neuroinflammation decreased with both doses of NCN. HFRD-induced decreases in serotonergic neurotransmission, 5-HT1A receptor expression, and morphological alterations in the rat's hypothalamus were normalized by both doses of NCN. In conclusion, NCN, as a potential antioxidant and neuromodulator, can normalize feeding behavior and produce antidepressant and memory-improving effects in a rat model of obesity following HFRD intake.
本研究调查了高脂饮食（HFRD）对使用烟酸（NCN）处理后的大鼠下丘脑进行行为、生化、神经化学和组织病理学研究的影响。将大鼠分为高脂饮食组和正常饮食（ND）组，并给予选定剂量的NCN，即25毫克/毫升/千克（低剂量）和50毫克/毫升/千克（高剂量），持续8周。雄性大鼠（n = 8）的分组如下：（i）溶剂对照组（Veh）+正常饮食；（ii）正常饮食+低剂量NCN；（iii）正常饮食+高剂量NCN；（iv）溶剂对照组+高脂饮食；（v）高脂饮食+低剂量NCN；（vi）高脂饮食+高剂量NCN。行为测试评估类似抑郁的症状和空间记忆；之后，分离下丘脑以对处死的动物进行各种分析。两种剂量的NCN均降低了两个饮食组的食物摄入量和生长速率，并表现出抗抑郁和增强记忆的作用。两种剂量的NCN均降低了高脂饮食诱导的氧化神经炎症。高脂饮食诱导的大鼠下丘脑血清素能神经传递减少、5-HT1A受体表达降低和形态学改变，均被两种剂量的NCN恢复正常。总之，作为一种潜在的抗氧化剂和神经调节剂，NCN可以使进食行为正常化，并在高脂饮食摄入后的肥胖大鼠模型中产生抗抑郁和改善记忆的作用。
