2,4-二氯苯氧基乙酸降解性质粒pJP4的遗传图谱和物理图谱
Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4.
作者信息
Don R H, Pemberton J M
出版信息
J Bacteriol. 1985 Jan;161(1):466-8. doi: 10.1128/jb.161.1.466-468.1985.
Abstract
Plasmid pJP4 is an 80-kilobase, IncP1, broad-host-range conjugative plasmid of Alcaligenes eutrophus encoding resistance to mercuric chloride and phenyl mercury acetate and degradation of 2,4-dichlorophenoxyacetic acid, 2-methyl-4-chlorophenoxyacetic acid, and 3-chlorobenzoate. By the use of cloning, transposon mutagenesis, and restriction endonuclease analysis, a biophysical and genetic map of pJP4 was generated.
摘要
质粒pJP4是产碱杆菌的一种80千碱基、IncP1型、广宿主范围的接合质粒，编码对氯化汞和苯基醋酸汞的抗性以及对2,4-二氯苯氧乙酸、2-甲基-4-氯苯氧乙酸和3-氯苯甲酸的降解能力。通过克隆、转座子诱变和限制性内切酶分析，构建了pJP4的生物物理图谱和遗传图谱。
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