Ghosal D, You I S, Chatterjee D K, Chakrabarty A M
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1638-42. doi: 10.1073/pnas.82.6.1638.
All of the structural genes for 3-chlorobenzoate degradation are clustered in a 4.2-kilobase (kb) region of plasmid pAC25 (or pAC27) in Pseudomonas putida. An approximate 10-kb DNA segment containing three structural genes for chlorocatechol metabolism present on plasmid pJP4 in Alcaligenes eutrophus shows homology with the above 4.2-kb region of pAC27. In spite of the detectable sequence homology in the structural genes present on both plasmids, the regulation of their expression seems quite different; unlike pAC27, structural rearrangements are prerequisite for efficient expression of the 3-chlorobenzoate genes on plasmid pJP4. Structural features such as stem-loop structures present on plasmid pJP4 are most likely the starting materials for such rearrangements.
恶臭假单胞菌中负责3-氯苯甲酸酯降解的所有结构基因都聚集在质粒pAC25（或pAC27）的一个4.2千碱基（kb）区域内。产碱杆菌中存在于质粒pJP4上的一个约10 kb的DNA片段，其中包含三个负责氯儿茶酚代谢的结构基因，与pAC27的上述4.2 kb区域具有同源性。尽管两种质粒上的结构基因存在可检测到的序列同源性，但它们的表达调控似乎有很大不同；与pAC27不同，结构重排是质粒pJP4上3-氯苯甲酸酯基因高效表达的先决条件。质粒pJP4上存在的茎环结构等结构特征很可能是此类重排的起始材料。
