Cheng Sirui, Tao Kezhu, Lv Xin, Lai Zhenduo, Lv Yi, Wang Zhixin, Chen Qinghe
School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication, Hainan University, Sanya 572025, China.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
J Agric Food Chem. 2025 Jan 8;73(1):329-341. doi: 10.1021/acs.jafc.4c09639. Epub 2024 Dec 16.
Oxathiapiprolin, a novel fungicide targeting oxysterol-binding proteins (OSBP), has proven to be effective against various oomycete diseases, but its effects on pathogens remain underexplored. In this study, we demonstrated that oxathiapiprolin inhibits mycelium growth, sporangium production, zoospore release, and oospore production in , with 50% effective concentrations ranging from 0.000153 to 0.011681 μg mL. Additionally, in vivo tests confirmed its efficacy in controlling litchi downy blight. Our study demonstrated that oxathiapiprolin disrupts cellular ultrastructure and plasma membrane integrity, affects stress sensitivity, inhibits sterol transport, increases autophagy levels, reduces lipid droplet content, and induces lipid metabolism disorders. Gene expression analysis revealed that oxathiapiprolin affects the expression of (ORP1 gene of ), with docking simulations further confirming its interaction with the PlORP1 (ORP1 protein of ) protein. Ultimately, this cascade of events led to pathogen death. This paper reveals the antioomycete mechanism of oxathiapiprolin against .
恶唑菌酮，一种靶向氧化甾醇结合蛋白（OSBP）的新型杀菌剂，已被证明对多种卵菌病害有效，但其对病原体的作用仍未得到充分研究。在本研究中，我们证明恶唑菌酮可抑制荔枝霜霉病菌的菌丝生长、孢子囊产生、游动孢子释放和卵孢子产生，其50%有效浓度范围为0.000153至0.011681μg/mL。此外，体内试验证实了其对荔枝霜霉病的防治效果。我们的研究表明，恶唑菌酮会破坏细胞超微结构和质膜完整性，影响胁迫敏感性，抑制甾醇运输，提高自噬水平，降低脂滴含量，并诱导脂质代谢紊乱。基因表达分析显示，恶唑菌酮会影响荔枝霜霉病菌的ORP1基因表达，对接模拟进一步证实了其与荔枝霜霉病菌ORP1蛋白的相互作用。最终，这一系列事件导致病原体死亡。本文揭示了恶唑菌酮对荔枝霜霉病菌的抗卵菌机制。
