Lu Ren-Yi, Ling Zhong-Yi, Chen Lin-Lin, Xu Wei-Heng, Xing Xin-Hao, Song Ze-Cheng, Chen Li, Wang Yan
School of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, 200433, China; The Center for Fungal Infectious Diseases Basic Research and Innovation of Medicine and Pharmacy, Ministry of Education, Shanghai, 200433, China.
School of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, 200433, China; School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
J Ethnopharmacol. 2025 Jan 31;340:119248. doi: 10.1016/j.jep.2024.119248. Epub 2024 Dec 15.
Dahuang Mudan decoction (DMD) is a traditional Chinese prescription from Zhang Zhongjing's Synopsis of the Golden Chamber. In clinical practice, it is often used in the treatment of infectious diseases.
To assess the therapeutic effect of DMD and its disassembled prescriptions on septic mice, and explore its potential mechanism.
Cecal ligation and puncture (CLP) sepsis and endotoxemia mice models were established. The effects of DMD, its disassembled prescriptions and active compounds were studied. Xuebijing injection (XBJ) was used as positive drug. Mice 7-day survival rates, blood biochemical markers, hematoxylin and eosin (HE) staining and immune cell infiltration were used to evaluate the overall protective effect of the drugs on mice. Inflammatory cytokines and coagulation activation indicators were detected by enzyme-linked immunosorbent assay (ELISA).
DMD, its Huoxue prescription, constituent drugs Mudanpi (MDP) and Taoren (TR) significantly protected mice with sepsis, improved the survival rate, reduced the degree of organ damage, and reduced the infiltration of immune cells in the lung tissues. The protective effect is comparable to that of XBJ. MDP and TR inhibited the levels of inflammatory factors and coagulation activation in septic mice. Paeonol and paeoniflorin in MDP showed significant protective effects on septic mice, and inhibited inflammatory cytokines level and coagulation activation.
These results confirm that DMD and its disassembled prescriptions have good therapeutic effect on septic mice, and the mechanism may be related to inhibition of the inflammatory response and coagulation activation.
大黄牡丹汤（DMD）是张仲景《金匮要略》中的一首传统中药方剂。在临床实践中，常用于治疗感染性疾病。
评估大黄牡丹汤及其拆方对脓毒症小鼠的治疗效果，并探讨其潜在机制。
建立盲肠结扎穿孔（CLP）脓毒症和内毒素血症小鼠模型。研究了大黄牡丹汤及其拆方和活性成分的作用。血必净注射液（XBJ）作为阳性对照药。通过小鼠7天生存率、血液生化指标、苏木精-伊红（HE）染色和免疫细胞浸润来评估药物对小鼠的整体保护作用。采用酶联免疫吸附测定（ELISA）检测炎性细胞因子和凝血激活指标。
大黄牡丹汤及其活血方、组成药物牡丹皮（MDP）和桃仁（TR）对脓毒症小鼠有显著保护作用，提高了生存率，减轻了器官损伤程度，减少了肺组织免疫细胞浸润。保护作用与血必净注射液相当。牡丹皮和桃仁抑制脓毒症小鼠炎性因子水平和凝血激活。牡丹皮中的丹皮酚和芍药苷对脓毒症小鼠有显著保护作用，并抑制炎性细胞因子水平和凝血激活。
这些结果证实大黄牡丹汤及其拆方对脓毒症小鼠有良好的治疗效果，其机制可能与抑制炎症反应和凝血激活有关。
