Meng Yan, Cai Xuan-Lin, Cong Shan, Sun Jiao, Hu Yong-Wei, Luo Li
Department of Rheumatology and Immunology, The First Affiliated Hospital at Xinjiang Medical University, Urumqi, People's Republic of China.
Graduate School, Xinjiang Medical University, Urumqi, People's Republic of China.
Cell Biochem Funct. 2024 Dec;42(8):e70026. doi: 10.1002/cbf.70026.
Rheumatoid arthritis (RA), a highly disabling autoimmune disease, is characterized by joint damage and synovial hyperplasia. This paper was designed to explore the effect and mechanisms of Aconitum diphtheria on the proliferation and apoptosis of RA fibroblast-like synoviocytes (RA-FLS). First, RA-FLS was treated with different doses of A. diphtheria extracts. Then, an inverted biological microscope was adopted to observe the RA-FLS morphology. Subsequently, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining, Cell Counting Kit-8 and western blot were utilized to analyze the apoptosis, proliferation, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway-related proteins in RA-FLS, respectively. The experimental results disclosed that, after treatment of RA-FLS with A. diphtheria extracts, the cells became round and the intercellular space was increased. Besides, the RA-FLS proliferation was effectively inhibited by A. diphtheria extracts, while the apoptosis was promoted. Additionally, A. diphtheria extracts could effectively downregulate the expression levels of p-NF-κB (p50), NF-κB (p50), p-NF-κB (p65), and NF-κB (p65). Collectively, A. diphtheria can effectively inhibit RA-FLS proliferation and promote apoptosis in a dose-dependent manner. Similarly, A. diphtheria is able to downregulate p-NF-κB and NF-κB protein expression, but there is no dose-response relationship.
类风湿性关节炎（RA）是一种高度致残的自身免疫性疾病，其特征为关节损伤和滑膜增生。本文旨在探讨白喉乌头对类风湿性关节炎成纤维样滑膜细胞（RA-FLS）增殖和凋亡的影响及机制。首先，用不同剂量的白喉乌头提取物处理RA-FLS。然后，采用倒置生物显微镜观察RA-FLS的形态。随后，分别利用末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记染色、细胞计数试剂盒-8和蛋白质免疫印迹法分析RA-FLS中的细胞凋亡、增殖以及活化B细胞核因子κB（NF-κB）信号通路相关蛋白。实验结果表明，用白喉乌头提取物处理RA-FLS后，细胞变圆，细胞间隙增大。此外，白喉乌头提取物可有效抑制RA-FLS的增殖，同时促进其凋亡。另外，白喉乌头提取物可有效下调p-NF-κB（p50）、NF-κB（p50）、p-NF-κB（p65）和NF-κB（p65）的表达水平。总体而言，白喉乌头能有效抑制RA-FLS增殖并以剂量依赖性方式促进其凋亡。同样，白喉乌头能够下调p-NF-κB和NF-κB蛋白表达，但不存在剂量反应关系。
