胶原酶组金属蛋白酶（MMP-1、MMP-8和MMP-13）在卵巢癌生化诊断中的诊断效用

Diagnostic Utility of Metalloproteinases from Collagenase Group (MMP-1, MMP-8 and MMP-13) in Biochemical Diagnosis of Ovarian Carcinoma.

作者信息

Kicman Aleksandra, Gacuta Ewa, Marecki Rafał, Kicman Michał Stanisław, Kulesza Monika, Klank-Sokołowska Ewa, Knapp Paweł, Niczyporuk Marek, Szmitkowski Maciej, Ławicki Sławomir

机构信息

Department of Aesthetic Medicine, The Faculty of Pharmacy, Medical University of Białystok, 15-267 Białystok, Poland.

Department of Perinatology, University Clinical Hospital of Bialystok, 15-276 Białystok, Poland.

出版信息

Cancers (Basel). 2024 Nov 26;16(23):3969. doi: 10.3390/cancers16233969.

BACKGROUND

Ovarian carcinoma (OC) has an unfavorable prognosis due to lack of screening and an asymptomatic course. New diagnostic methods are being sought to enable earlier diagnosis of this condition. The purpose of this study was to determine the diagnostic utility of collagenases (MMP-1, MMP-8 and MMP-13) in the diagnosis of OC compared to HE4 and CA125 and the ROMA.

METHODS

The study group consisted of 120 patients with OC, the control group: 70 patients with benign ovarian lesions (BLs) and 50 healthy women (HS). MMP-1, MMP-8 and MMP-13 were determined by ELISA and HE4 and CA125 by CMIA.

RESULTS

OC patients had higher levels of MMP-1 and MMP-13 compared to the BL and HS groups. MMP-1 (SE: 81.66%; SP: 94%; PPV: 97.02%; NPV: 68.11%; AUC: 0.9625) and MMP-13 (SE: 77.50%; SP: 94%; PPV: 96.875%; NPV: 63.51%; AUC: 0.917) showed similar or higher diagnostic values to routine markers (HE4: SE:85%; SP: 92%; PPV: 96.22%; NPV: 71.875%; AUC: 0.943; CA125: SE: 80%; SP: 98%; PPV: 98.96%; NPV: 67.12%; AUC: 0.909) and the ROMA (SE: 90.83%; SP: 94%; PPV: 97.32%; NPV: 81.03%; AUC: 0.955). Performing combined analyses of individual MMPs and MMPs with ROMA was associated with further increases in diagnostic parameters.

CONCLUSIONS

MMP-1 and MMP-13 have shown preliminary potential as diagnostic markers and auxiliary markers to ROMA in biochemical diagnosis of OC.

背景

由于缺乏筛查且病程无症状，卵巢癌（OC）的预后不佳。目前正在寻求新的诊断方法以实现对该疾病的早期诊断。本研究的目的是确定与HE4、CA125和ROMA相比，胶原酶（MMP-1、MMP-8和MMP-13）在OC诊断中的应用价值。

方法

研究组由120例OC患者组成，对照组包括70例卵巢良性病变（BL）患者和50例健康女性（HS）。通过ELISA法测定MMP-1、MMP-8和MMP-13，通过CMIA法测定HE4和CA125。

结果

与BL组和HS组相比，OC患者的MMP-1和MMP-13水平更高。MMP-1（敏感性：81.66%；特异性：94%；阳性预测值：97.02%；阴性预测值：68.11%；曲线下面积：0.9625）和MMP-13（敏感性：77.50%；特异性：94%；阳性预测值：96.875%；阴性预测值：63.51%；曲线下面积：0.917）显示出与常规标志物（HE4：敏感性：85%；特异性：92%；阳性预测值：96.22%；阴性预测值：71.875%；曲线下面积：0.943；CA125：敏感性：80%；特异性：98%；阳性预测值：98.96%；阴性预测值：67.12%；曲线下面积：0.909）和ROMA（敏感性：90.83%；特异性：94%；阳性预测值：97.32%；阴性预测值：81.03%；曲线下面积：0.955）相似或更高的诊断价值。对单个MMP以及MMP与ROMA进行联合分析与诊断参数的进一步提高相关。