Ewa Gacuta, Ławicki Paweł, Grabowska Hanna, Ławicki Michał, Kulesza Monika, Kicman Aleksandra, Malinowski Paweł, Ławicki Sławomir
Department of Perinatology, University Clinical Hospital of Bialystok, 15-276 Bialystok, Poland.
Department of Population Medicine and Lifestyle Diseases Prevention, The Faculty of Medicine, Medical University of Białystok, 15-269 Bialystok, Poland.
Int J Mol Sci. 2025 Jun 13;26(12):5661. doi: 10.3390/ijms26125661.
Ovarian cancer (OC) has an extremely unfavourable prognosis. This is due to its asymptomatic course and lack of screening tests. Therefore, new methods are needed to diagnose OC. The aim of this study was to evaluate the concentrations and diagnostic utility of selected matrilysins and stromelysins in the diagnosis of OC in comparison with the classical markers CA125 and HE4. The study group included 100 patients with serous OC, 70 with serous cysts (BL), and 50 healthy women (HW). Selected MMPs were determined by ELISA, routine markers by CMIA. Ovarian cancer patients have elevated concentrations of MMP-7, MMP-26, MMP-10 as well as CA125 and HE4 in the total group and subgroups (stage I + II, and III + IV). The highest values of diagnostic parameters-SP, SE, NPV, PPV, and ACC, as compared to CA125 and HE4, were observed for MMP-7. Performing ROC analyses showed that the highest AUC values were observed for MMP-7, CA125, and HE4, in the whole group of patients and divided into stages I and II according to FIGO. Performing ROC analyses for groups III and IV according to FIGO was associated with an increase in AUC for the MMPs studied. Of the MMPs tested, MMP-7, MMP-26, and MMP-10 have the highest potential in diagnostics of serous ovarian cancer patients.
卵巢癌（OC）的预后极差。这是由于其无症状病程以及缺乏筛查检测手段。因此，需要新的方法来诊断OC。本研究的目的是评估所选基质溶解素和基质溶素在OC诊断中的浓度及诊断效用，并与经典标志物CA125和HE4进行比较。研究组包括100例浆液性OC患者、70例浆液性囊肿患者（BL）和50名健康女性（HW）。通过酶联免疫吸附测定（ELISA）测定所选基质金属蛋白酶（MMPs），通过化学发光微粒子免疫分析（CMIA）测定常规标志物。在整个组以及亚组（I + II期和III + IV期）中，卵巢癌患者的MMP-7、MMP-26、MMP-10以及CA125和HE4浓度均升高。与CA125和HE4相比，MMP-7的诊断参数——特异度（SP）、灵敏度（SE）、阴性预测值（NPV）、阳性预测值（PPV）和准确度（ACC）最高。进行ROC分析表明，在整个患者组以及根据国际妇产科联盟（FIGO）分为I期和II期的患者中，MMP-7、CA125和HE4的曲线下面积（AUC）值最高。对根据FIGO分为III期和IV期的组进行ROC分析，所研究的MMPs的AUC增加。在所测试的MMPs中，MMP-7、MMP-26和MMP-10在浆液性卵巢癌患者的诊断中具有最高潜力。
