Erzar Eva, Tzankov Alexandar, Ocvirk Janja, Grčar Kuzmanov Biljana, Boltežar Lučka, Prevodnik Veronika Kloboves, Gašljević Gorana
Department of Cytopathology, Institute of Oncology Ljubljana, Zaloška Cesta 2, 1000 Ljubljana, Slovenia.
Medical Faculty, University of Ljubljana, Vrazov Trg 2, 1000 Ljubljana, Slovenia.
Cancers (Basel). 2024 Nov 29;16(23):4011. doi: 10.3390/cancers16234011.
BACKGROUND/OBJECTIVES: The tumour microenvironment (TME) plays an important role in the development and progression of cancer and it differs among lymphomas, both with respect to the composition and quantity of specific tumour-infiltrating immune cells (TICs), such as FoxP3 regulatory T cells (Tregs). The role of FoxP3 Tregs in the TME of peripheral T-cell lymphomas (PTCLs) is complex, and their impact on overall survival (OS) remains unclear. Consequently, we aim to evaluate and compare the FoxP3 cell quantity in nodal PTCLs and reactive lymph nodes (LNs), with a focus on investigating their impact on OS.
excisional lymph node (LN) biopsies from 105 nodal PTCLs and 17 reactive LNs are immunohistochemically stained for FoxP3. Visual scoring of FoxP3 cells is performed, and different cut-off values are used to evaluate the impact of FoxP3 cell quantity on OS.
FoxP3 cells are present in the TME of all cases, except for four cases where FoxP3 is expressed in lymphoma cells. Lower FoxP3 cell quantities are observed in certain nodal PTCL subtypes compared to reactive LNs. Patients with high FoxP3 cell quantities show improved OS. However, the FoxP3 cell quantity is not confirmed as an independent prognostic biomarker.
these findings underscore the promise of FoxP3 cell quantities as added value in prognosis and highlight the potential benefits of Treg-stimulating therapies in PTCLs.
背景/目的：肿瘤微环境（TME）在癌症的发生和发展中起重要作用，并且在淋巴瘤之间存在差异，无论是在特定肿瘤浸润免疫细胞（TIC）的组成和数量方面，例如FoxP3调节性T细胞（Tregs）。FoxP3 Tregs在外周T细胞淋巴瘤（PTCL）的TME中的作用是复杂的，它们对总生存期（OS）的影响仍不清楚。因此，我们旨在评估和比较淋巴结PTCL和反应性淋巴结（LN）中的FoxP3细胞数量，重点是研究它们对OS的影响。
对105例淋巴结PTCL和17例反应性LN的切除淋巴结活检组织进行FoxP3免疫组织化学染色。对FoxP3细胞进行视觉评分，并使用不同的临界值来评估FoxP3细胞数量对OS的影响。
除4例淋巴瘤细胞中表达FoxP3的病例外，所有病例的TME中均存在FoxP3细胞。与反应性LN相比，在某些淋巴结PTCL亚型中观察到较低的FoxP3细胞数量。FoxP3细胞数量高的患者显示出更好的OS。然而，FoxP3细胞数量未被确认为独立的预后生物标志物。
这些发现强调了FoxP3细胞数量在预后方面作为附加值的前景，并突出了Treg刺激疗法在PTCL中的潜在益处。
