Yu Baisheng, Li Hangfan, Chen Qiong, Yang Chuang, Guo Yongqing, Sun Baoli
College of Animal Science, South China Agriculture University, Guangzhou 510642, China.
Animals (Basel). 2024 Nov 24;14(23):3379. doi: 10.3390/ani14233379.
This study employed high-throughput sequencing to explore bacterial diversity and hematological variations across different age groups of giraffes, as well as the impact of diarrhea on their intestinal microbiota. Additionally, the correlation between intestinal flora and hematological indices was examined for the first time. Firmicutes, Bacteroides, and Proteobacteria were the predominant bacterial groups in the giraffe's intestinal flora. The α-diversity analysis indicated significant variations in microbial diversity among giraffes of varying ages ( < 0.05). Furthermore, giraffes suffering from diarrhea exhibited significant alterations in the abundance of Proteobacteria and Actinobacteriota at the phylum level ( < 0.05). At the genus level, , , and had significant differences compared to healthy counterparts ( < 0.05). Hematological parameters such as lymphocyte count (Lym), red blood cell count (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), and plateletcrit (PCT) varied significantly across different age groups ( < 0.05). A substantial correlation was observed between the intestinal microbiome composition and hematological parameters ( < 0.05). In conclusion, this study highlights significant differences in both the intestinal microbiome composition and hematological indices among giraffes of different ages. Diarrhea was found to significantly alter the abundance and composition of the intestinal microbial community. These insights provide a valuable theoretical foundation for the microbiological and hematological aspects of giraffe health management and breeding practices.
本研究采用高通量测序技术，探究不同年龄组长颈鹿的细菌多样性和血液学变化，以及腹泻对其肠道微生物群的影响。此外，首次检测了肠道菌群与血液学指标之间的相关性。厚壁菌门、拟杆菌门和变形菌门是长颈鹿肠道菌群中的主要细菌类群。α多样性分析表明，不同年龄的长颈鹿之间微生物多样性存在显著差异（<0.05）。此外，患有腹泻的长颈鹿在门水平上变形菌门和放线菌门的丰度发生了显著变化（<0.05）。在属水平上，与健康的长颈鹿相比，[此处原文缺失具体属名]、[此处原文缺失具体属名]和[此处原文缺失具体属名]存在显著差异（<0.05）。淋巴细胞计数（Lym）、红细胞计数（RBC）、平均红细胞体积（MCV）、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）、血小板计数（PLT）和血小板压积（PCT）等血液学参数在不同年龄组之间存在显著差异（<0.05）。观察到肠道微生物群组成与血液学参数之间存在显著相关性（<0.05）。总之，本研究突出了不同年龄长颈鹿在肠道微生物群组成和血液学指标方面的显著差异。发现腹泻会显著改变肠道微生物群落的丰度和组成。这些见解为长颈鹿健康管理和繁殖实践的微生物学和血液学方面提供了有价值的理论基础。
