Dou Xufeng, Zhang Guodong, Tang Hao, Chen Xiaoxue, Chen Beibei, Mei Yuxia, Jiao Haihong, Ren Min
Key Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin, Alar 843300, China.
College of Life Science and Technology, Tarim University, Alar 843300, China.
Animals (Basel). 2024 Dec 1;14(23):3470. doi: 10.3390/ani14233470.
Carotenoids from different sources have different structures and functions, and their dietary components benefit the health of various organisms. The effects of halophilic Archaea-derived C50 carotenoids on poultry egg quality and gut microbiota remain largely unexplored. In this study, we isolated a carotenoid-secreting strain of , TRM89021, from the Pamir Plateau. We characterized the carotenoid pigments produced by this strain; the major components were bacterioruberin and its derivatives. The effects of these carotenoids on the egg quality and cecal microbiota composition of hens were investigated. Compared to the basal diet group (BDG), supplementation with carotenoids in the carotenoids-supplemented diet group (CDG) resulted in significantly lower a* and b* scores at week 5 and lower b* scores and Haugh units at week 2, while egg strength and weight were higher. CDG also showed increased yolk antioxidant capacity, higher glutathione peroxidase levels, and significantly lower catalase levels ( < 0.05). Plasma analysis revealed elevated total bilirubin and aspartate aminotransferase levels, along with reduced inorganic phosphorus levels in the CDG ( < 0.05). No significant differences in cecal microbiota diversity were observed between the groups at any taxonomic level. This result suggests that halophilic archaea-derived carotenoids have the potential to be used as natural feed supplements to improve egg quality. Our study provides a theoretical basis for applying archaea-derived carotenoids in poultry diets.
来自不同来源的类胡萝卜素具有不同的结构和功能，其膳食成分有益于各种生物体的健康。嗜盐古菌衍生的C50类胡萝卜素对禽蛋品质和肠道微生物群的影响在很大程度上仍未得到探索。在本研究中，我们从帕米尔高原分离出一株产类胡萝卜素的菌株TRM89021。我们对该菌株产生的类胡萝卜素色素进行了表征；主要成分是细菌红素及其衍生物。研究了这些类胡萝卜素对母鸡蛋品质和盲肠微生物群组成的影响。与基础日粮组（BDG）相比，类胡萝卜素补充日粮组（CDG）在第5周时a和b评分显著降低，在第2周时b*评分和哈夫单位降低，而蛋强度和重量更高。CDG的蛋黄抗氧化能力也有所提高，谷胱甘肽过氧化物酶水平更高，过氧化氢酶水平显著降低（P<0.05）。血浆分析显示，CDG中的总胆红素和天冬氨酸转氨酶水平升高，无机磷水平降低（P<0.05）。在任何分类水平上，两组之间的盲肠微生物群多样性均未观察到显著差异。这一结果表明，嗜盐古菌衍生的类胡萝卜素有可能用作天然饲料添加剂来改善蛋品质。我们的研究为将古菌衍生的类胡萝卜素应用于家禽日粮提供了理论依据。
