Tian Yong, Li Guoqin, Zhang Shuo, Zeng Tao, Chen Li, Tao Zhengrong, Lu Lizhi
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Science, Hangzhou, China.
Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs of China, Hangzhou, China.
Front Microbiol. 2022 Sep 30;13:955115. doi: 10.3389/fmicb.2022.955115. eCollection 2022.
Fermented plant product (FPP) is a kind of functional complex containing probiotics and a variety of bioactive substances, which has multiple physiological functions. However, there is no systematic appraisal of FPP as a feed additive for laying hens. This study was conducted to evaluate the utilization of FPP in laying hens. A total of 120 healthy 34-week-old Xianju layers with similar body weight and egg production were randomly allocated into two dietary treatments with four replicates per treatment and 15 birds per replicate for 8 weeks. The dietary treatments included the basal diet without FPP (CON group) and CON diet supplemented with 500 mg/kg of FPP (FPP group). Compared with the CON group, the egg production and egg mass were significantly increased in the FPP group from 38 to 42 and 34 to 42 weeks of age ( < 0.05). Birds fed with the diet containing 500 mg/kg FPP had higher albumen height ( < 0.01) and Haugh unit ( < 0.05) than those of the controls. FPP supplementation significantly increased the villus height (VH) and crypt depth (CD) in the jejunum of laying hens ( < 0.01), as well as the ratio of VH to CD ( < 0.05). The mRNA expression of tight junctions showed that dietary supplementation with FPP significantly increased the expression levels of ( < 0.01) and ( < 0.05) in jejunum of hens compared to the control group. In addition, dietary supplementation with FPP influenced cecal microbiota of laying hens, which was characterized by the changes in the microbial community composition, including the increased abundances of , and , along with the decreased abundance of , and . Spearman's correlation analysis revealed that bacteria such as , and were significantly and positively correlated with the intestinal barrier markers ( < 0.05), with extremely significant correlations between and , and and ( < 0.01), whereas had a negative correlation with the expression of ( < 0.05). As it can be concluded, FPP supplementation increased the egg production, egg mass, albumen height, and Haugh unit of laying hens, and improved intestinal health by ameliorating intestinal barrier function, which may be partially attributed to the regulation of cecal microbiota. Our findings suggest that FPP has the potential to be used as a feed additive to promote the performance of layers.
发酵植物产品（FPP）是一种含有益生菌和多种生物活性物质的功能性复合物，具有多种生理功能。然而，目前尚无对FPP作为蛋鸡饲料添加剂的系统评价。本研究旨在评估FPP在蛋鸡中的利用情况。将120只体重和产蛋量相近的34周龄健康仙居蛋鸡随机分为两种日粮处理组，每组4个重复，每个重复15只鸡，试验期8周。日粮处理包括不添加FPP的基础日粮（CON组）和添加500mg/kg FPP的CON日粮（FPP组）。与CON组相比，FPP组在38至42周龄和34至42周龄时的产蛋量和蛋重显著增加（P<0.05）。饲喂含500mg/kg FPP日粮的蛋鸡的蛋白高度（P<0.01）和哈氏单位（P<0.05）均高于对照组。添加FPP显著增加了蛋鸡空肠的绒毛高度（VH）和隐窝深度（CD）（P<0.01），以及VH与CD的比值（P<0.05）。紧密连接的mRNA表达显示，与对照组相比，日粮添加FPP显著增加了母鸡空肠中ZO-1（P<0.01）和Occludin（P<0.05）的表达水平。此外，日粮添加FPP影响了蛋鸡的盲肠微生物群，其特征是微生物群落组成发生变化，包括双歧杆菌属、乳酸杆菌属和阿克曼菌属丰度增加，以及大肠杆菌、肠球菌属和拟杆菌属丰度降低。Spearman相关性分析表明，双歧杆菌属、乳酸杆菌属和阿克曼菌属等细菌与肠道屏障标志物显著正相关（P<0.05），其中双歧杆菌属与ZO-1、乳酸杆菌属与Occludin之间呈极显著相关（P<0.01），而大肠杆菌与Occludin的表达呈负相关（P<0.05）。综上所述，添加FPP提高了蛋鸡的产蛋量、蛋重、蛋白高度和哈氏单位，并通过改善肠道屏障功能改善了肠道健康，这可能部分归因于对盲肠微生物群的调节。我们的研究结果表明，FPP有潜力作为饲料添加剂来提高蛋鸡的生产性能。
