Suriyasathaporn Witaya, Kongkaew Aphisek, Intanon Montira, Srithanasuwan Anyaphat, Saipinta Duanghathai, Pangprasit Noppason, Thongtharb Atigan, Chuasakhonwilai Areerat, Chaisri Wasana
School of Veterinary Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.
Research Center of Producing and Development of Products and Innovations for Animal Health and Production, Chiang Mai University, Chiang Mai 50100, Thailand.
Animals (Basel). 2024 Dec 6;14(23):3526. doi: 10.3390/ani14233526.
The microbial ecology in mastitis involves the interactions between bacteria and the mammary gland environment. Poor mastitis control, for which understanding these microbial relationships is crucial, increases the risk of mastitis and co-infections. The aim of this study was to determine the pathogenesis and bacterial ecology of murine mammary glands following intramammary infection (IMI) with (AU), (SA), and four isolates of selected non-aureus staphylococci (NAS), as well as co-infections of AU or SA with NAS. Selected NAS demonstrated resistance to growth inhibition after co-culture with AU and SA and were proven to be protective in vitro against AU and SA. Both single infections and co-infections of AU, SA, two selected isolates of (NAS1 and NAS2), and two selected isolates of (NAS3 and NAS4) were injected into the murine mammary glands at 10 CFU/mL. After 72 h of inoculation, the results showed that AU, AU-NAS2, and AU-NAS3 expressed severe inflammation with clinical signs of mastitis. The persistence of AU was found for AU, NAS3, and AU-NAS3, whereas the persistence of SA was found for SA-NAS1, SA-NAS3, and SA-NAS, although single SA could not persist. Interestingly, NAS2 in both the single- and co-IMIs with AU or SA resulted in a spontaneous cure 72 h after the IMI. In conclusion, some NAS have beneficial results because they can eliminate and , but some NAS have negative effects because they can support persistent infection. These results may be used to explain both the advantages and disadvantages of NAS on farms with poor mastitis control. In addition, the beneficial may initiate natural live antibiotics and reduce antibiotic resistance problems.
乳腺炎中的微生物生态学涉及细菌与乳腺环境之间的相互作用。对于乳腺炎控制不佳而言，了解这些微生物关系至关重要，而这会增加乳腺炎和混合感染的风险。本研究的目的是确定用金黄色葡萄球菌（AU）、表皮葡萄球菌（SA）和四种选定的非金黄色葡萄球菌（NAS）分离株进行乳腺内感染（IMI）后，小鼠乳腺的发病机制和细菌生态学，以及AU或SA与NAS的混合感染情况。选定的NAS在与AU和SA共培养后表现出对生长抑制的抗性，并在体外被证明对AU和SA具有保护作用。将AU、SA、两种选定的凝固酶阴性葡萄球菌分离株（NAS1和NAS2）以及两种选定的腐生葡萄球菌分离株（NAS3和NAS4）以10 CFU/mL的浓度分别或混合注射到小鼠乳腺中。接种72小时后，结果显示AU、AU-NAS2和AU-NAS3表现出严重炎症并有乳腺炎的临床症状。发现AU、NAS3和AU-NAS3中存在AU的持续感染，而在SA-NAS1、SA-NAS3和SA-NAS中发现了SA的持续感染，尽管单一的SA无法持续存在。有趣的是，在与AU或SA的单重和混合IMI中，NAS2在IMI后72小时导致自发治愈。总之，一些NAS有有益的结果，因为它们可以消除AU和SA，但一些NAS有负面影响，因为它们可以支持AU的持续感染。这些结果可用于解释在乳腺炎控制不佳的农场中NAS的优缺点。此外，有益的NAS可能会启动天然的活菌抗生素并减少抗生素耐药性问题。
