Wang Tao, Tian Zhe, Yu Mubin, Zhang Shuer, Zhang Min, Zhai Xiangwei, Shen Wei, Wang Junjie
College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China.
General Station of Animal Husbandry of Shandong Province, Jinan 250100, China.
Animals (Basel). 2024 Dec 8;14(23):3546. doi: 10.3390/ani14233546.
There is a consensus that indigenous pigs in China are more resistant than modern commercial pigs in terms of disease resistance. Generally, the immune response is an important part of anti-disease capability; however, the related mechanism in pigs is largely puzzling. Here, the public transcriptome data of peripheral blood mononuclear cells (PBMCs) from Dapulian (Chinese local breed) and Landrace (Commercial breed) pigs after stimulation with polyinosinic-polycytidylic acid (poly I:C, a conventional reagent used for simulation of the viral infection) were reanalyzed, and the immune response mechanism in different pig breeds was investigated from a transcriptomic perspective. Of note, through comparative analyses of Dapulian and Landrace pigs, the candidate genes involved in swine broad-spectrum resistance were identified, such as , and . In addition, after differential gene expression, target gene identification and functional enrichment analyses, a potential regulatory network of miRNA genes associated with immune response was obtained in Dapulian pigs, including five miRNAs and 12 genes (such as ssc-miR-181a, ssc-miR-486, and ). This work provides new insights into the immune response regulation of antiviral responses in indigenous and modern commercial pigs.
人们普遍认为，中国本土猪在抗病能力方面比现代商业猪更强。一般来说，免疫反应是抗病能力的重要组成部分；然而，猪的相关机制在很大程度上仍不清楚。在此，对来自大蒲莲猪（中国地方品种）和长白猪（商业品种）的外周血单个核细胞（PBMC）在聚肌苷酸-聚胞苷酸（poly I:C，一种用于模拟病毒感染的传统试剂）刺激后的公共转录组数据进行了重新分析，并从转录组学角度研究了不同猪品种的免疫反应机制。值得注意的是，通过对大蒲莲猪和长白猪的比较分析，鉴定出了参与猪广谱抗性的候选基因，如 、 和 。此外，在进行差异基因表达、靶基因鉴定和功能富集分析后，在大蒲莲猪中获得了一个与免疫反应相关的潜在miRNA基因调控网络，包括5个miRNA和12个基因（如ssc-miR-181a、ssc-miR-486、 和 ）。这项工作为本土猪和现代商业猪抗病毒反应的免疫反应调控提供了新的见解。
