College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China; College of Life Sciences, Key Laboratory of Animal Reproduction and Biotechnology in Universities of Shandong, Qingdao Agricultural University, Qingdao 266109, China.
Rural Agriculture Bureau of Chengyang District, Qingdao 266109, China.
Gene. 2022 Aug 5;834:146649. doi: 10.1016/j.gene.2022.146649. Epub 2022 Jun 6.
Porcine circovirus type 2 (PCV2) has been a notorious killer for the pig industry, causing substantial economic losses worldwide. However, its pathogenesis is still poorly understood. Comparative transcriptomic analysis and weighted gene co-expression network analysis (WGCNA) were performed in different porcine tissues after PCV2 infection. Our comparative transcriptomic analysis obtained 40 key differentially expressed genes (DEGs), and our WGCNA identified 458 hub genes. Significantly, both TPX2 microtubule nucleation factor (TPX2) and Aurora kinase A (AURKA) are included in these key DEGs and hubs genes. Our gene ontology (GO) analysis indicated that the key DEGs and hub genes participated in cell cycle regulation and immune response. The expressive levels of TPX2 and AURKA went down in the spleen but up in the kidneys after infection with PCV2. We conclude that TPX2 and AURKA played an essential role in PCV2 infection.
猪圆环病毒 2 型（PCV2）一直是养猪业的致命杀手，在全球范围内造成了巨大的经济损失。然而，其发病机制仍不清楚。本研究在 PCV2 感染后对不同猪组织进行了比较转录组分析和加权基因共表达网络分析（WGCNA）。我们的比较转录组分析获得了 40 个关键差异表达基因（DEGs），我们的 WGCNA 鉴定了 458 个枢纽基因。值得注意的是，微管核形成因子（TPX2）和 Aurora 激酶 A（AURKA）均包含在这些关键 DEGs 和枢纽基因中。我们的基因本体（GO）分析表明，关键 DEGs 和枢纽基因参与细胞周期调控和免疫反应。PCV2 感染后，脾脏中 TPX2 和 AURKA 的表达水平下降，而肾脏中则上升。我们得出结论，TPX2 和 AURKA 在 PCV2 感染中发挥了重要作用。
