Ferguson Ross, Subramanian Vasanta
Department of Life Sciences, University of Bath, Building 4 South, Bath BA2 7AY, UK.
Cells. 2024 Nov 25;13(23):1957. doi: 10.3390/cells13231957.
Talpid3 is a basal body protein required for the formation of primary cilia, an organelle involved in signal transduction. Here, we asked if Talpid3 has a role in the regulation of differentiation and/or self-renewal of ES cells and whether cells lacking cilia due to a deletion in Talpid3 can be reprogrammed to induced pluripotent stem (iPS) cells. We show that mouse embryonic limb fibroblasts which lack primary cilia with a targeted deletion in the () gene can be efficiently reprogrammed to iPS cells. Furthermore, vector-free iPS cells retain ES cell features and are able to self-renew. However, both iPS and ES cells are unable to form visceral endoderm and differentiate poorly into neurons. The observed defects are not a consequence of reprogramming since ES cells also exhibit this phenotype. Thus, Talpid3 and primary cilia are required for some differentiation events but appear to be dispensable for stem cell self-renewal and reprogramming.
Talpid3是一种形成初级纤毛所必需的基体蛋白，初级纤毛是一种参与信号转导的细胞器。在此，我们探究Talpid3是否在胚胎干细胞的分化和/或自我更新调控中发挥作用，以及由于Talpid3缺失而缺乏纤毛的细胞是否能够重编程为诱导多能干细胞（iPS细胞）。我们发现，在（）基因中存在靶向缺失从而缺乏初级纤毛的小鼠胚胎肢体成纤维细胞能够高效重编程为iPS细胞。此外，无载体iPS细胞保留了胚胎干细胞的特征并且能够自我更新。然而，iPS细胞和胚胎干细胞都无法形成内胚层，并且向神经元的分化能力较差。观察到的缺陷并非重编程的结果，因为胚胎干细胞也表现出这种表型。因此，Talpid3和初级纤毛对于某些分化事件是必需的，但对于干细胞自我更新和重编程似乎是可有可无的。
