Lipilkina Tatiana Alexandrovna, Xu Cristhian, Barbosa Matheus de Souza, Khramova Valentina Nikolaevna, Shebeko Sergei K, Ermakov Alexey M, Ivanova Iskra Vitanova, Todorov Svetoslav Dimitrov
ProBacLab, Laboratório de Microbiologia de Alimentos, Departamento de Alimentos e Nutrição Experimental, Food Research Center, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo 05508-000, Brazil.
Faculty of Bioengineering and Veterinary Medicine, Don State Technical University, Gagarina Sq., 1, Rostov-on-Don 344002, Russia.
Foods. 2024 Nov 25;13(23):3770. doi: 10.3390/foods13233770.
This work aimed to evaluate some of the probiotic features and safety of the bacteriocin-producing subsp. 2a. The effect of selected commercial drugs from different generic groups and antibiotics on the growth of subsp. 2a was also determined. The presence of virulence factors was determined based on PCR with total DNA from subsp. 2a. Good growth of subsp. 2a was recorded in MRS broth supplemented with 0.2% or 0.4% oxbile or in MRS broth adjusted to a pH from 5.0-9.0. Auto-aggregation of subsp. 2a was 62.59%. Different levels of co-aggregation were recorded between subsp. 2a and ATCC19443, ATCC15521 and ScottA. Growth of subsp. 2a was not inhibited by commercial drugs from different generic groups. The inhibitory effect on the growth of subsp. 2a was recorded only in the presence of Arotin [selective serotonin reuptake inhibitor antidepressant] Minimal Inhibition Concentration (MIC) 1.0 mg/mL, Atlansil [Antiarrhythmic] MIC 0.625 mg/mL, Diclofenac potassium [non-steroidal anti-inflammatory drug (NSAID)] MIC 2.5 mg/mL and Spidufen [NSAID] MIC 15.0 mg/mL. Only two antibiotics tested in this study, Amoxil and Urotrobel, inhibited the growth of subsp. 2a with a MIC of <0.5 mg/mL and 5.0 mg/mL, respectively. However, subsp. 2a generated positive PCR results on the DNA level for A (vancomycin resistance), (hyaluronidase), (enterococcal surface protein), (adhesion of collagen) and A (cytolisin) and a high virulence profile when examined for the presence of virulence factors. It is important to underline that cytolysis has been described as a virulence and antibacterial factor.
本研究旨在评估产细菌素的亚种2a的一些益生菌特性和安全性。还测定了来自不同通用类别的选定商业药物和抗生素对亚种2a生长的影响。基于对亚种2a总DNA进行的PCR检测来确定毒力因子的存在。在添加0.2%或0.4%牛胆汁的MRS肉汤中或pH值调节至5.0 - 9.0的MRS肉汤中，亚种2a生长良好。亚种2a的自聚集率为62.59%。在亚种2a与ATCC19443、ATCC15521和ScottA之间记录到了不同程度的共聚集。来自不同通用类别的商业药物未抑制亚种2a的生长。仅在存在阿罗汀[选择性5-羟色胺再摄取抑制剂抗抑郁药]时记录到对亚种2a生长的抑制作用，其最低抑菌浓度（MIC）为1.0毫克/毫升，阿特兰西尔[抗心律失常药]MIC为0.625毫克/毫升，双氯芬酸钾[非甾体抗炎药（NSAID）]MIC为2.5毫克/毫升，斯皮杜芬[NSAID]MIC为15.0毫克/毫升。本研究中仅测试的两种抗生素，阿莫西林和乌洛托品，分别以<0.5毫克/毫升和5.0毫克/毫升的MIC抑制了亚种2a的生长。然而，当检测毒力因子的存在时，亚种2a在DNA水平上针对A（耐万古霉素）、（透明质酸酶）、（肠球菌表面蛋白）、（胶原黏附）和A（溶细胞素）产生了阳性PCR结果，且具有高毒力特征。需要强调的是，细胞溶解已被描述为一种毒力和抗菌因子。
