Yu Xi, Wang Xiaotian, Liu Xintong, Li Fangfei, Bao Yihong, Chai Yangyang
School of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province, Harbin 150040, China.
Foods. 2024 Nov 29;13(23):3868. doi: 10.3390/foods13233868.
Diarrheal irritable bowel syndrome (IBS-D) is a chronic bowel condition that leads to intestinal dysfunction and is typically accompanied by diarrhea, stomach pain, and abdominal distension. L. polyphenols (RNPs), which are natural plant polyphenols, are the subject of this study, which aims to assess their potential in improving IBS-D and to explore the underlying mechanisms through a network pharmacology analysis and 16S rRNA sequencing. Next, mice models of diarrhea-predominant irritable bowel were established, and the mice with IBS-D were treated with RNPs. The effect of RNPs was then evaluated in terms of body weight, abdominal withdrawal reflex (AWR), Bristol score, fecal water percentage, diluted fecal volume, total intestinal transit time, immune index, histopathological observation, and changes in inflammatory factors. Finally, 16S rRNA sequencing and reverse q-RTPCR were utilized to evaluate the components that mediate the impact of RNPs on IBS-D. It was found that when RNP treatment was administered to mice with IBS-D, they decreased the water content in their stools, raised their immunological scores, and decreased the amount of inflammatory substances in their bodies. Moreover, through 16S rRNA sequencing, it was shown that the RNP treatment increased the relative abundances of Bacteroides, Alloprevotella, and Alistipes, which led to the remodeling of gut microbiota. In summary, RNPs significantly improved the conditions of mice with IBS-D by inhibiting the FoxO pathway and enhancing gut microbiota. This study concludes that RNPs could significantly improve the symptoms of mice with IBS-D through these means.
腹泻型肠易激综合征（IBS-D）是一种导致肠道功能障碍的慢性肠道疾病，通常伴有腹泻、胃痛和腹胀。本研究的对象是天然植物多酚——L. 多酚（RNPs），旨在评估其改善IBS-D的潜力，并通过网络药理学分析和16S rRNA测序探索其潜在机制。接下来，建立腹泻型肠易激综合征小鼠模型，并用RNPs治疗IBS-D小鼠。然后从体重、腹部退缩反射（AWR）、布里斯托大便分类法评分、粪便含水量、稀释粪便体积、肠道总转运时间、免疫指标、组织病理学观察以及炎症因子变化等方面评估RNPs的效果。最后，利用16S rRNA测序和逆转录q-RTPCR评估介导RNPs对IBS-D影响的成分。研究发现，给IBS-D小鼠施用RNP治疗后，它们的粪便含水量降低，免疫评分提高，体内炎症物质含量减少。此外，通过16S rRNA测序表明，RNP治疗增加了拟杆菌属、别普雷沃菌属和阿里斯杆菌属的相对丰度，从而导致肠道微生物群重塑。总之，RNPs通过抑制FoxO通路和增强肠道微生物群，显著改善了IBS-D小鼠的状况。本研究得出结论，RNPs可通过这些方式显著改善IBS-D小鼠的症状。
